Ying‑Chu Lin1 , Cheng‑Feng Tsai1,2* and Hsiao‑Ling Huang3*
Abstract
Background The effects of a low concentration of hypochlorous acid (HOCl) mouthwash on salivary bacteria remained unclear. We aimed to evaluate the antibacterial effects of 100 ppm HOCl mouthwash on salivary bacteria, including Staphylococcus aureus (S. aureus), in patients with periodontal disease (PD).
Methods Patients with PD were randomized into mouthwash-only (MW, n=26) and mouthwash with periodon‑tal flosser (MWPF, n=27) groups. Patients without PD were selected for the control group (n=30). S. aureus culture and saliva samples (before and after the intervention) were collected for bacterial DNA extraction. A real-time poly‑ merase chain reaction assay and serial dilutions of S. aureus culture and saliva samples were used to measure the salivary bacteria total count (SBTC) and confirm the antibacterial effects of the mouthwash using S. aureus.
Results No significant difference in demographic data was observed among the three groups. Before the intervention, the baseline SBTC of the MW and MWPF groups was significantly higher than that of the control group. After the mouthwash rinses, the SBTC data significantly changed in the MW and MWPF groups only (by 62.4% and 77.4%, respectively). After the base-2 log-transformation of the SBTC data, a similar trend was observed. Linear regression revealed that baseline SBTC and the MWPF intervention significantly affected SBTC reduction percentage by volume. After incubation with 10% (v/v) of mouthwash, the survival rates of 106 and 107 colony-forming units/mL of S. aureus were 0.51%±0.06% and 1.42%±0.37%, respectively.
Conclusions These study results indicated that 100 ppm HOCl mouthwash treatment could effectively reduce SBTC in patients with PD and the abundance of S. aureus. It provides that the HOCl mouthwash can be an option for individuals to help control SBTC, especially in patients with PD.
Trial registration The study protocol was approved by the Institutional Review Board of Kaohsiung Medical University Hospital (KMUHIRB-F(I)-20200042) on 20/03/2020 and retrospectively registered at ClinicalTrial.gov (NCT05372835) on 13/05/2022.
Keywords Antibacterial ability, Total bacterial count, Hypochlorous acid, Mouthwash, Intervention, Bacterial growth estimation
张 燕１，朱 迪１，孙 磊１，王任贤２
（１．徐州仁慈医院感染管理科，江苏 徐州 ２４１００４；２．台湾防疫学会，台湾 台中 ４０４４７）
［摘 要］ 目的 探讨次氯酸应用于外科术后切口愈合及减少术后切口感染的临床效果。方法 纳入某三级甲等医院足踝外科住院患者，按手术时序进行分组：第一阶段（２０２４年１０月１６日—１２月１６日）为对照组，采用４５００～５５００ｍｇ／Ｌ碘伏消毒术后切口；第二阶段（２０２４年１２月１７日—２０２５年２月１７日）为试验组，采用（１１０±１６．５）ｍｇ／Ｌ次氯酸消毒术后切口。术后第１、３、５、７、１０、１４天进行切口消毒、指标评价并记录。主要结局指标为手术部位感染，次要指标包括术后４８ｈ红肿、过敏及疼痛程度评分。结果 对照组感染发病率为０．９６％，术后４８ｈ红肿发生率为１６．９９％，疼痛评分（１．４１±０．４９）分；试验组未发生感染，术后４８ｈ红肿发生率为９．５８％，疼痛评分为（１．３６±０．４８）分。两组感染发病率及疼痛评分差异均无统计学意义（均 犘＞０．０５），红肿发生率差异具有统计学意义（犘＝０．００６）。按切口类型进行分层分析显示，开放性外伤亚组中，对照组感染发病率为３．１９％，红肿发生率为２９．７９％，疼痛评分为（１．６２±０．４９）分；试验组术后４８ｈ红肿发生率为８．１４％，疼痛评分为（１．４１±０．４９）分，两组感染发病率差异无统计学意义（犘＞０．０５），红肿发生率（犘＜０．００１）及疼痛程度评分（犘＝０．００５）差异均有统计学意义。闭合性外伤亚组中，各结局指标组间比较差异均无统计学意义（均犘＞０．０５）。结论 次氯酸溶液在闭合性外伤术后切口应用的效果与碘伏相当；在开放性外伤术后应用中，其控制术后红肿发生及疼痛的效果优于碘伏。
［关 键 词］ 次氯酸；碘伏；骨科；切口愈合；实效性随机对照试验
［中图分类号］ Ｒ１８１．３＋２ Ｒ１８７＋ ．１
Authors
Julia Danne
Stella Gwini
Dean McKenzie
Vickie R. Driver
Alexander Reyzelman
Jill Kawalec
Michael French
Sevinc Sahin
Keywords
pilonidal sinus
Kara S. Couch
CWS; Lisa Corbett
CWOCN; Lisa Gould
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
扫一扫了解详情:
任何关于疾病的建议都不能替代执业医师的面对面诊断。所有门诊时间仅供参考，最终以医院当日公布为准。
网友、医生言论仅代表其个人观点，不代表本站同意其说法，请谨慎参阅，本站不承担由此引起的法律责任。
