Chaiyawat Aonsri 1,2 , Sompop Kuljarusnont 3

and Duangjai Tungmunnithum 4,5,*

1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

2 Unit of Compounds Library for Drug Discovery, Mahidol University, Bangkok 10400, Thailand

3 Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

4 Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand

5 Le Studium Institute for Advanced Studies, 1 Rue Dupanloup, 45000 Orléans, France 

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。; Tel./Fax: +66-26448696

Academic Editors: Lina Raudone,˙ Mindaugas Liaudanskas and Sonata Trumbeckaite

Received: 8 January 2025

Revised: 20 February 2025

Accepted: 24 February 2025

Published: 26 February 2025

Citation: Aonsri, C.; Kuljarusnont, S.; Tungmunnithum, D. Discovering Skin Anti-Aging Potentials of the Most Abundant Flavone Phytochemical Compound Reported in Siam Violet Pearl, a Medicinal Plant from Thailand by In Silico and In Vitro Assessments. Antioxidants 2025, 14, 272. https://doi.org/10.3390/ antiox14030272

Copyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).

Abstract: Currently, nutraceuticals and functional food/cosmeceutical sectors are seeking natural molecules to develop various types of phytopharmaceutical products. Flavonoids have been reported in antioxidant and many medical/pharmacological activities. Monochoria angustifolia or Siam violet pearl medicinal plant is the newest species of the genus Monochoria C. Presl, which have long been consumed as food and herbal medicines. Though previous work showed that apigenin-7-O-glucoside is the most abundant antioxidant phytochemical found in this medicinal plant, the report on anti-aging activity is still lacking and needs to be filled in. The objective of this work is to explore anti-aging capacities of the most abundant antioxidant phytochemical reported in this plant using both in silico and in vitro assessments. In addition, pharmacokinetic properties were predicted. Interestingly, the results from both in silico and in vitro analysis showed a similar trend that apigenin-7- O-glucoside is a potential anti-aging agent against three enzymes. The pharmacokinetic properties, such as adsorption, distribution, metabolism, excretion and toxicity (ADMET), of this compound are also provided in this work. The current study is also the first report on anti-aging properties of this Thai medicinal plant. However, the safety and efficacy of future developed products from this compound and clinical study should be determined in the future.

Keywords: flavone; Monochoria angustifolia; flavonoids; medicinal plants; anti-aging; molecular modeling; pharmacological activity; medical benefits

Yijia Zhang1 | Xueer Wang1 | Jianyuan Huang1 | Xinyue Zhang1 | Lingwei Bu1 |

Yarui Zhang1 | Fengting Liang1 | Shenhua Wu1 | Min Zhang1 | Lu Zhang2 |

Lin Zhang1

1 GDMPA Key Laboratory of key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China

2 Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Center for Orthopaedic Surgery of the Third Affiliated Hospital, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Southern Medical University, Guangzhou, China

Correspondence

Lu Zhang, Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Center for Orthopaedic Surgery of the Third Affiliated Hospital, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Southern Medical University, Guangzhou, China.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 Lin Zhang, GDMPA Key Laboratory of key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Funding information

National Natural Science Foundation of China, Grant/Award Number: 82073417, 81872514 and 81971297; GDMPA Key Laboratory Project of Scientific and Technological Innovation, Grant/Award

Number: 2023ZDZ12; Guangdong Basic and Applied Basic Research Foundation, Grant/Award Number: 2022A1515010768, 2023A1515012480 and 2024A121301233

Abstract

Skin aging has been associated with the onset of various skin issues, and recent studies have identified an increase in Cdc42 activity in naturally aging mice. While previous literature has suggested that CASIN, a specific inhibitor of Cdc42 activity, may possess anti-aging properties, its specific effects on the epidermis and dermis, as well as the underlying mechanisms in naturally aging mice, remain unclear. Our study revealed that CASIN demonstrated the ability to increase epidermal and dermal thickness, enhance dermal-epidermal junction, and stimulate collagen and elastic fiber synthesis in 9-, 15-, and 24-month-old C57BL/6 mice in vivo. Moreover, CASIN was found to enhance the proliferation, differentiation, and colony formation and restore the cytoskeletal morphology of primary keratinocytes in naturally aging skin in vitro. Furthermore, the anti-aging properties of CASIN on primary fibroblasts in aging mice were mediated by the ribosomal protein RPL4 using proteomic sequencing, influencing collagen synthesis and cytoskeletal morphology both in vitro and in vivo. Meanwhile, both subcutaneous injection and topical application exhibited anti-aging effects for a duration of 21 days. Additionally, CASIN exhibited anti-inflammatory properties, while reduced expression of RPL4 was associated with increased inflammation in the skin of naturally aging mice. Taken together, our results unveil a novel function of RPL4 in skin aging, providing a foundational basis for future investigations into ribosomal proteins. And CASIN shows promise as a potential anti-aging agent for naturally aging mouse skin, suggesting potential applications in the field.

KEYWORDS

CASIN, Cdc42, fibroblast, keratinocyte, ribosome, skin aging

Qin Zhang1, Dangdang Cheng1,2 & FeifeiWang3

This study aimed to develop in vivo methods for assessing facial anti-glycation and anti-aging effects and to investigate the link between glycation and aging signs. We utilized an AGE reader to measure AGEs levels on the face and arms, establishing a correlation to validate the reader’s use for facial AGEs detection. Then the product’s 7-day anti-glycation effect was evaluated. And its 56-day anti-aging effects were evaluated using non-invasive probes and VISIA CR, assessing skin tone (skin brightness L* and yellowness b*, skin gloss), texture (skin elasticity R2 and firmness F4, skin pores), and hydration (skin moisture content). Correlations between facial AGEs levels and aging parameters were analyzed.

Results indicated a strong correlation between facial and arm AGEs after product application, supporting facial AGEs level as an assessment parameter. The product demonstrated substantial antiglycation and anti-aging effects, suggesting the methods’ efficacy for cosmetic evaluation. A stronger overall correlation was found between AGEs levels and skin tone than with skin texture and hydration, highlighting glycation’s impact on skin color.

Keywords AGE reader, Anti-glycation evaluation, Anti-aging evaluation, Glycation-aging correlation

Patrick Bogdanowicz 1,4囚 , Paul Bensadoun 2,4, Maïté Noizet 1 , Benoît Béganton 1 , Armony Philippe 1 , SandrineAlvarez‑Georges 1 , Gautier Doat 3 , AmélieTourette 1 , Sandrine Bessou‑Touya 1 , Jean‑Marc Lemaitre 2囚 & Hélène Duplan 1

1 R&D Pierre Fabre Dermo-Cosmétique & Personal Care, Toulouse, France.

2 INSERM IRMB UMR1183, Hôpital Saint Eloi, Université de Montpellier, Montpellier, France.

3 Laboratoires Dermatologiques Avène, Lavaur, France. 4These authors contributed equally: Patrick Bogdanowicz and Paul Bensadoun.

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Intrinsic and extrinsic factors, including lifestyle and sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead to skin aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) that includes MMPs, CXCLs and S100A8/9. Reducing the SASP with senotherapeutics is a promising strategy to reduce skin aging. Here we evaluated the effect of a formula containing niacinamide and hyaluronic acid, which are known to limit senescence and skin aging. We conducted three different studies. (1) Ex vivo explants treated with the formula had more collagen and glycosaminoglycan. (2) In a clinical trial with forty-four women, two months of treatment improved fne lines, wrinkles, luminosity, smoothness, homogeneity, and plumpness. (3) In a third study on thirty women, we treated one arm for two months and took skin biopsies to study gene expression. 101 mRNAs and 13 miRNAs were differentially expressed. We observed a likely senomorphic efect, as there was a decrease in many SASP genes including MMP12 and CXCL9 and a significant downregulation of autocrine signaling genes: S100A8 and S100A9. These pharmaco-clinical results are the first to demonstrate the senomorphic properties of an effective anti-aging formula in skin.