Suradwadee Thungmungmee1 , Boonchoo Sritularak2 , Nakuntwalai Wisidsri1 , Nattakan Kanana3 , Nattika Nimmano3
1 Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand;
2 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok, Thailand;
3 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Correspondence: Nattika Nimmano, Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, Tel +66 0 2188399, Fax +66 0 2188401, Email 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Purpose: The study aimed to investigate in vitro anti-aging activities of 29 Dendrobium spp. and develop and characterize microemulsions (MEs) for topical application.
Methods: Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated.
Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2′,7′- dichlorofluorescin diacetate (DCFDA) assays, respectively. D. kentrophyllum extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.
Results: D. kentrophyllum appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and antielastase activities. The extract was safe for human skin cells at a concentration of 6.25–100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.
Conclusion: DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.
Keywords: reactive oxygen species, anti-aging activities, Dendrobium kentrophyllum, microemulsion
Magdalena Wójciak 1,* , Piotr Drozdowski 2,* , Agnieszka Skalska-Kami ´nska 1 , Martyna Zagórska-Dziok 3 , Aleksandra Ziemlewska 3 , Zofia Nizioł-Łukaszewska 3 and Małgorzata Latalska 4
1 Department of Analytical Chemistry, Medical University of Lublin, Chod´zki 4a, 20-093 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
2 Department of Plastic Surgery, Specialist Medical Centre, 57-320 Polanica-Zdrój, Poland
3 Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.Z.-D.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.Z.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Z.N.-Ł.)
4 Department of General and Pediatric Ophthalmology, Medical University of Lublin, 20-079 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.W.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (P.D.)
Citation: Wójciak, M.; Drozdowski, P.; Skalska-Kami ´nska, A.; Zagórska Dziok, M.; Ziemlewska, A.; Nizioł- Łukaszewska, Z.; Latalska, M. Protective, Anti-Inflammatory, and Anti-Aging Effects of Soy Isoflavones on Skin Cells: An Overview of In Vitro and In Vivo Studies. Molecules 2024, 29, 5790. https://doi.org/10.3390/ molecules29235790
Academic Editors: José Basilio Heredia, Luis Angel CabanillasBojórquez and Marilyn
Shomara Criollo-Mendoza
Received: 1 November 2024
Revised: 1 December 2024
Accepted: 5 December 2024
Published: 7 December 2024
Abstract: Isoflavones are found in numerous plant species within the Leguminosae family; however, soy isoflavones are particularly significant in practice and have been extensively studied in recent years. The health-promoting potential of orally administered soy isoflavones is widely documented in the scientific literature, and many review articles have been developed to highlight their significance. However, it should be noted that soy-isoflavone-rich extracts and isolated soy isoflavones, such as genistein and daidzein, are also often applied topically as ingredients in many formulations, including face creams, tonics, and emulsions. New delivery systems are continuously being developed to enhance the skin permeability of isoflavones, thus improving their efficacy. In this context, their direct activity on skin cells is an important aspect of scientific research. The anti-inflammatory, protective, and antioxidant properties of isoflavones and soy extracts make them promising cosmetic ingredients with anti-aging potential because inflammation and the accumulation of reactive oxygen species (ROS) can lead to structural and functional changes in skin cells, accelerating the aging process. This review provides an overview of research on the impact of the application of soy isoflavone extract and soy-derived isoflavones on skin cells, with a focus on the documented molecular mechanisms underlying their effects. This study aims to offer essential insights to aid in the development of functional cosmetics and future clinical applications.
Keywords: phytoestrogens; topical application; genistein; daidzein; fibroblasts; keratinocytes; skin
擅长大面积深度烧伤的早期救治、各种烧烫伤创面的综合处理、烧伤后瘢痕增生挛缩畸形的修复。长期致力于急性皮肤缺损、慢性皮肤溃疡（褥疮、糖尿病足等）的外科治疗。
擅长领域：介入微创诊疗血管性疾病等，如下肢动脉硬化闭塞症、糖尿病足、血管畸形、静脉曲张、动静脉血栓形成、静脉狭窄闭塞等。
东莞康华医院国际造口治疗师，采用全球倡导的伤口湿性愈合疗法和造口专科护理新技术，在糖尿病溃疡足、下肢动/静脉溃疡、骨髓炎、外科术后感染/脂肪液化、肿瘤伤口、放射性皮炎、痛风破溃感染、压力性损伤（压疮）、液体外渗、造口并发症、失禁、瘘管等方面提供专业化的诊治、咨询及健康教育。
从事骨科临床工作和外科学教学工作30年。擅长骨关节疾病、四肢骨折和手外伤的保守治疗、手术治疗（关节置换手术、四肢骨折内固定手术、创伤修复手术）；各类创面的修复：包括糖尿病足、难治性痛风破溃感染伤口、创伤术后伤口不愈合。
长期从事影像诊断和介入放射学诊疗工作，擅长肿瘤和血管病的介入治疗。
佛山市禅城区中心医院慢性伤口造口专科主任。
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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