罗高兴 刘梦龙

陆军军医大学（第三军医大学）第一附属医院全军烧伤研究所，创伤、烧伤与复合伤国家重点实验室，重庆市疾病蛋白质组学重点实验室 400038

通信作者：罗高兴 Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

【摘要】皮肤创面是临床常见病症之一。功能材料通过结构调节和性能整合，可以针对性地对创面进行保护并促进创面愈合，目前已在创面修复领域得到广泛应用，是临床创面治疗的重要工具之一。本文分别就止血类、抗菌类、抗炎类、促血管化类及调控创面微环境类功能材料在创面修复中的应用做一总结。

【关键词】皮肤；伤口愈合；功能材料；创面微环境

基金项目： 国家自然科学基金重点国际合作项目(81920108022)；国家自然科学基金重点项目(81630055)

Application of functional materials to promote cutaneous wound healing
   Luo Gaoxing, Liu Menglong
      State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing Key Laboratory for Disease Proteomics, Chongqing 400038, China Corresponding author: Luo Gaoxing, Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

[ Abstract ] Cutaneous wound is one of the common clinical diseases. Functional materials can provide targeted wound protection and promote wound healing through the structural adjustment and functional integration. Currently, functional materials have been widely used in the field of wound repair, becoming one of the important tools for clinical wound treatment. This paper summarizes the application of functional
materials of following categories including hemostasis,antibacterial, anti-inflammation, vascularization, and regulation of wound microenvironment in wound repair.

[ Key words ] Skin; W ound healing; Functional materials; W ound microenvironment

Fund program: Key International Cooperation Program of National Natural Science Foundation of China (81920108022); Key Program of N ational Natural Science Foundation of China (81630055).

金 岩F! 刘 源 *! 张 超*! 董 蕊*! 雷 娟*

（第四军医大学组织工程研发中心， 西安 710032

摘要： 目 的 利 用 自 体 表 皮 干 细 胞 与 异 体 角 膜 基 质 细 胞 在 体 外 构 建 双 层 组 织 工 程 角 膜 ! 并 修 复 兔 角 膜 缘 干 细 胞 的 缺 损 " 方 法 建 立 兔 角 膜 缘 干 细 胞 缺 损 模 型 ! 以 去 细 胞 猪 角 膜 基 质 片 作 为 支 架 材 料 ! 以 自 体 表 皮 干 细 胞 与 异 体 角 膜 基 质 细 胞 作 为 种 子 细 胞 ! 在 体 外 构 建 组 织 工 程 角 膜 ! 并 用 来 修 复 兔 角 膜 缘 干 细 胞 缺 损 " 结 果 利 用 自 体 表皮 干 细 胞 与 异 体角 膜 基 质 细 胞 复合 异 种 去 角 膜 基 质 片 在 体 外 成 功 构 建 组 织 工 程 角 膜 # 构 建 的 组 织 工 程 角 膜 与 正 常 角膜 相 似 ! 具 有 上 皮层 和 基 质 层 # 用 组 织 工 程 角 膜 修 复 兔 角 膜 缘 干 细 胞 缺 损 T 个 月 后 ! 损 伤 角 膜 透 明 度 恢 复 良 好 ! 组 织 学 结 构基 本 恢 复 正 常 " 结论 成 功 构 建 了 兔 的双 层 组 织 工 程 眼角 膜 ! 并 修 复 了 兔角 膜 缘 干 细 胞 缺损 "

关键词" 表皮干细胞# 角膜# 组织工程# 角膜损伤

中图分类号：R 775.2

文献标识码：2

文章编号: 1000-503X(2005)06-0674-04

Treatment of Rabbit Corneal W ounds with Skin Epidermal Stem Cells

Jin Yan*, Liu Yuan*, Zhang Chao*, Dong Rui*, Lei Juan*

(Research Center for Tissue Engineering, the Fourth Military Medical University, Xi'an 710032, China)

Abstract: Objective To construct artificial rabbit corneas with autologous skin epidermal stem cells and allogenic stromal cells in vitro and promote healing of corneal wounds.

Methods: Skin epidermal stem cells were isolated from autologous skin samples. Keratocytes were isolated from newborn cornea biopsies. The cells were combined with acelular porcine corneal stroma scaffold to construct artificial corneas. Then the constructed artificial corneas were used to repair severe vision loss caused by complete loss of corneal epithelial stem cells.

Results: Cultured skin epidermal stem cells and keratocytes were in good growth conditions.Cultured artificial corneas consisted of multiplayer epithelial cells growing on stroma equivalent consisting of stromal matrix with incorporated keratocytes. The in vitro constructed artificial corneas were histologically similar to normal rabbit corneas. Three months after transplantation, the cornea wounds were healed and the rabbit cornea became transparent.

Conclusion: The artificial corneas were constructed successfully in vitro and can be used to repair severe vision loss caused by complete loss of corneal epithelial stem cells.

Key words: epidermal stem cells; cornea; tissue engineering; comeal wound Acta Acad Med Sin, 2005 ,27(6):674-677

Acta Acad Med Sin, 2005 ,27(6):674-677

吕国忠 朱宇刚 周红梅 顾在秋 杨敏杰

[摘要]
目的探讨自.异体表皮细胞悬液混合移植技术在创而修复中的应用、

方法 30只，大鼠随机配成15对后.分成细胞悬液移植组(A组,10对)和细胞膜片移植组(B组,5对)。取每只大鼠的去全厚皮创面分离表皮细胞，并根据配对情况按1:1的细胞比例混合,体外常规培养。4 d后收获A组混合细胞悬液,14 d后收获B组混介细胞膜片、将此细胞悬液和膜片分别转移至A、B组相应供体大鼠的去全厚皮创面、随后A组每对大鼠的创面交叉覆益配对方的体全厚皮;B组创血覆盖胶原膜及“优妥”敷料。比较移梢后2~3周两组的创面修复情况。

结果 术后2~3 尚,A组创面大多愈介,表面光滑.与皮下连接紧密。术后第5天,B组创面部分细胞膜片脱落,部分成活。膜片成活的
创面后期再次出现小创面经久不愈，

结论 自异体表皮细胞悬液混合移植足一种可行的、体内构建皮肤、修复创面的方法。

[关键词] 表皮;细胞培养; 皮肤移植;烧伤

An experimental study on the in vivo intermingled culture of rat autologous and allogeneic epithelial cells LV Cuozhong, ZHU Yugang, ZHOU Hongmei. Department of Burns, The Third People' s Hospital, Wuxi 214041,Jiangsu Province, P. R. China

[ Abstract] Objective To explore a method for in vivo skin reconstruction. Methods Thirty Spraque-Dawley ( SD) rats were randomly divided into two paired groups, i. e. in vivo culture ( A group，10
pairs) and in vitro grafting (B group,5 pairs). Skin samples were harvested from the rats of the two groups for the isolation of epithelial cells which were then mixed cultured in vitro in 1: I ratio. Mixed cellular sus- pension in A group was harvested 4 days after culture. The mixed cellular sheets were harvested 14 days after culture. The cultured cells and sheets were then thansplanted onto ttal skin loss wounds of donor rats for further cultivation. The wounds in A group were covered with allogeneic full-thickness skin. While the wounds in group were covered by collagen membrane and gauze. Wound repair was observed and compared between the two groups al 2-3 post - operative weeks.

Results
Most of the wounds in A group healed after 2-3 weeks with smooth surface, and the peithelium connected closely and tightly with the: subcutaneous tissue. In the wounds in B group on 5 post-operative day . some of the cellular sheets survived and some fell off. Even the healed wounds in B group would be injured again resulting in protracted small wounds.

Conclusion In vivo in situ epithelia! culture might be an optional method of skin reconstruction for wound healing.

[Key words] Epithelium; Cell culture; Skin graft; Burm