西藏红缨合耳菊提取物的美白机理研究(一）

摘 要
西藏红缨合耳菊(Synotis  erythropappa (Bur.et Franch)C. Jeffrey et Y. L. Chen)为菊科(Compositae)植物，属西藏特有植物。产于西藏东南部，西藏当地女性将红缨合耳菊捣碎、烧成灰等后,敷在皮肤表面用于皮肤保养及紫外线防护。黑色素的大量产生是皮肤变黑的重要原因，人体中酪氨酸酶是催化酪氨酸形成多巴，以及多巴形成多巴醌，并最终产生黑色素的关键酶。羟自由基是人体内产生的不稳定的且具有强氧化性的物质，与人体的衰老有密切的联系，氧自由基同样对人体的衰老有密切关系,同时它也在黑色素的形成过程中起到氧化的作用。当自由基大量产生，又得不到有效的清除时，会导致机体组织不断老化。

本文通过研究红缨合耳菊提取物的美白机理，包括红缨合耳菊提取物对酪氨酸酶的抑制作用、B16细胞的影响、自由基清除作用等，探讨了红缨合耳菊在美白方面的功效。取得的结果如下:

一、以酪氨酸为底物，探讨红缨合耳菊提取物酪氨酸酶单酚酶的抑制效果结果表明红缨合耳菊提取物的乙酸乙酯及正丁醇萃取部分有较强的抑制能力，对酪氨酸酶单酚酶的半数抑制浓度IC₅₀分别为57.8、140_ug/mL。并能使抑制过程产生延迟作用。

二、以多巴为底物探讨红缨合耳菊提取物酪氨酸酶双酚酶的抑制效果。结果表明红缨合耳菊提取物的乙酸乙酯及正丁醇萃取部分有较强的抑制能力，对双酚酶的半数抑制浓度IC₅₀分别为41.2、59.6_ug/mL。通过动力学实验得出抑制类型分别为竞争性抑制以及非竞争性抑制。

三、探讨了红缨合耳菊提取物对B16细胞的影响，结果表明红缨合耳菊提取物对细胞形态和生长均有一定的抑制作用，其中以乙酸乙酯萃取部分最大320_ug/mL时细胞生长抑制率可达到65.67%,而正丁醇萃取部分及水溶部分仅为10%左右。

四、红缨合耳菊提取物在抗氧化方面的研究,探讨红缨合耳菊提取物对DPPH和氧自由基的清除作用量。对羟自由基的清除作用在一定程度上可以与抗坏血酸相媲美。乙酸乙酯、正丁醇萃取部分半数清除率IC₅₀分别为2.51、5.12_ug/mL与抗坏血酸的ICso值2.42_μg/mL相差不多，对氧自由基的半数抑制浓度IC50分为0.27、0.39 _mg/mL.

上述实验证明红缨合耳菊提取物具有抑制酪氨酸酶单酚酶及双酚酶的作用对细胞有一定的毒性，此外还具有清除自由基、抗氧化损伤、抗衰老的双重功效有望用于美白护肤和抗衰老型化妆品中。

关键词:红缨合耳菊;酪氨酸酶;B16黑色素瘤细胞;自由基

Abstract
Synotis erythropappa (Bur.et Franch.)C, Jeffrey et Y.L. Chen is Compositae plant, as a natural plant of Tibet. They mainly product in east-nouth of Tibet. The females apple the triturate or ash of them to cure skin disease, Also to avid eultraviolet radiation. As we known, the product of melanin is responsible for the process of skin blackening. Besides, tyrosinase is a key enzyme which can catalyze tyrosine to L-DOPA, and catalyze L-DOPA to dioxyphenylalanine quinine.

Free radicals, a matter produced in vivo, which is close connection with ageing.is instable but powerful in oxidation, If plenty of the free radicals produced with out efficient cleaning, the prganism will be ageing uninterrupt, This paper do some research on the effect of the extract of Synotis erythropappa (Bur. et Franch.)C Jeffrey et Y,L. Chen on the cosmetology,Bl6 cells and antioxygen, From the rsearch of the effect on the inhibition ratio of the tyrosinase and the cleaning of freradicals, we get the following results:

The research of water extract of Synotis erythropappa (Bur. et Franch.)C. Jeffrey et Y.L. Chen on monophenol oxidase of tyrosinase. We discover that the four factions extracted all have inhibitory activities on tyrosinase, inhibitory activity of ethyl acetate and n-butanol fraction are higher than others, concentration that resultsin 50% activity(ICso) lost is 57.8、140 μg/mL, Also they Can extend the lag of the action time of monophenol oxidase.

The research of water extract of Synotis erythropappa (Bur. et Franch.)C Jefrey et Y L. Chen on diphenol oxidase of tyrosinase. We discover that the four fractions extracted all have inhibitory activities on tyrosinase, inhibitory activity of ethyl acetate and n-butanol fraction are higher than others, concentration that resultsin 50% activity(ICso) lost is 41l.2、59.6 μg/mL, The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate faction is a competitive type inhibitor, and the inhibition constants for free enzyme(K¡) was determined to be19.7_μg/mL. n-butanol fraction is a uncompetitive type inhibitor, and the inhibition constants for free enzyme(K¡) was determined to be 60.7 _μg/mL.

The research of extract from Synotis erythropappa(Bur. et Franch.)C. Jeffrey et Y,L. Chen on Bl6 cells. We discover that the four fractions extracted all have inhibitory activities on B16 cells, inhibitory activity of ethyl acetate fractionare higher than others, ICso is 65.67%.

The research of extract of Synotis erythropappa (Bur. et Franch.) C. Jeffreyet Y, L. Chen on free radicals. We discover that the four fractions extracted all haveinhibitory activities on free radicals, inhibitory activity of ethyl acetate and n-butanol fraction are higher than others, IC₅₀ is 2.51、5.12 g/ml on DPPH and 0.27、0.39mg/mL on oxygen free radicals.
The above-mentioned research demonstrates that Synotis erythropappa (Bur. etFranch,)C, Jeffrey et Y,L. Chen has the effect on skin whitening anf anti-ageing. Soit is promising be used in skin protection cosmetics.

Key word: Synotis erythropappa (Bur. et Franch.)C. Jeffrey et Y. L. Chen; tyrosinase: Bl6 cell; free radicals.

光声电治疗术后皮肤黏膜屏障修复专家共识

中华医学会医学美容学会激光美容学组，中华医学会皮肤性病学会美容激光学组
[关键词】 皮肤黏膜修复,光声电治疗术后;共识
[中图分类号】 R751.05[文献标识码】Bdoi:10.16761/i.cnki.1000-4963.2019.05.019
[文章编号]1000-4963 2019)05-0319-04

西藏红缨合耳菊提取物对酪氨酸酶的抑制作用

徐学涛1，张：l昆h，杜志云’，郑劭1，方岩雄1，卫星船1，赵肃清1，俞新华2(1．广东工业大学轻工化工学院，广东广州510006；2．华南农业大学生命科学学院，广东广州510642)

摘要目的：研究西藏红缨合耳菊提取物对酪氨酸酶的抑制作用。方法：将红缨合耳菊的70％乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取。然后测定各部分对酪氨酸酶的抑制作用，并进行酶抑制动力学研究。结果：乙酸乙酯、正丁醇的萃取部分对酪氨酸酶的活性抑制较强；对单酚酶抑制作用明显并有延迟作用，半数抑制浓度(IC50)分别为57．8、140 pg／ml；对双酚酶的抑制作用显示浓度依赖关系，IC50分别为4I．2、59．6∥lIll，相比熊果苷抑制双酚酶活性的Ic，。值1．44rag／ml，抑制效果分别是熊果苷的35、24倍；抑制类型：乙酸乙酯部分为竞争性抑制，晒为19．7斗g／mJ，正丁醇部分为非竞争性抑制，鼬为60．7t∥ml。结论：西藏红缨合耳菊乙醇提取物的乙酸乙酯、正丁醇萃取部分对酪氨酸酶活性抑制较强。

关键词红缨合耳菊；酪氨酸酶；化妆品

中图分类号：R285．5

文献标识码：A

文章编号：1001掣54(2008)10-1544-04

Inhibition of Extracts from Synotis erythropappa OR Tyrosinase XU Xue·ta01，ZHANG Kunl，DU Zhi—yunl，ZHENG Jie。，FANG Yan．xiong‘，WE／Xing-ehuanl，ZHAO Su．qing。，YU Xin．hna2

(1．Faculty of Chemical Engineering and Light Industry，Guangdong University of Technology，Guangzhou 510006，China；2．College of Life Science，South China Agricultural University，Guangzhou 510642，China)

Abstract

Objectire：To study the inhibition of extracts from Synotis erythropappa On tyrosinase．Methods：The 70％ethanol ex． tracts were extracted by petroleum benzine，ethyl acetate and n-butanol，and the inhibitory activities against tyrosinase of every fraction were determined in vitro and the inhibitory kinetics of ethyl acetate and n-butanol fractions we地investigated．Results：The four fractions extracted all had inhibitory activities on tyrosinase。inhibitory activities of ethyl acetate and n-butanol fraction were higher．Their IC50 were 57．8、140 t,#ml for monophenol oxidase activity and 41．2、59．6 w旷IIll for diphenol oxidase activity，respectively．The inhibition kinetics analyzed by Hnewaver—Bunk plots showed that ethyl acetate fraction was a competitive type inhibitor，and its Ki Was de—termined to be 19．7 pe,／mL n·butanol fraction was an uncompetitive type inhibitor，and its Ki Was determined to be 60．7¨∥m1．Conclusion：Ethyl acetate and n—butanol fractions of extracts from Synotis erythropappa show potential inhibitory activity on tymsinase．

Key words

Synot／s erythropappa(Bur．et France)C．Jeffrey et Y．L Chen；Tyrosinase；Cosmetic

光声电治疗术后皮肤黏膜屏障修复专家共识

中华医学会医学美容学会激光美容学组，中华医学会皮肤性病学会美容激光学组

[关键词] 皮肤黏膜修复,光声电治疗术后;共识[中图分类号1R751.05[文献标识码]B

文章编号]1000-4963 2019)05-0319-04

doi:10.16761/i.enki.1000-4963.2019.05.019

中国年龄相关性黄斑变性临床诊疗指南 （2023年）

中华医学会眼科学分会眼底病学组 中国医师协会眼科医师分会眼底病学组

通信作者：许迅，上海交通大学医学院附属第一人民医院眼科 国家眼部疾病临床医学

研究中心 上海市眼底病重点实验室 上海眼视觉与光医学工程技术研究中心 上海市眼科疾病精准诊疗工程技术研究中心，上海 200080，Email：该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

【摘要】　年龄相关性黄斑变性（AMD）是老年人群低视力乃至失明的主要原因，随着我国人口老龄化加剧，AMD的患病人数将持续上升。近10年，眼底影像学检查技术飞速发展，为AMD的分型、诊断和随访提供了新的视角和途径，新药的开发为 AMD尤其新生血管性 AMD提供了更为多样的干预和治疗方法，而强调精准个体化治疗对眼底病医师提出了更高要求。为此，中华医学会眼科学分会眼底病学组、中国医师协会眼科医师分会眼底病学组与国家眼部疾病临床医学研究中心基于最新循证医学证据，结合国际指南和我国社会经济发展现状，围绕 8个临床问题提出推荐意见，形成了我国的AMD临床诊疗指南，以期加强和完善我国AMD诊疗工作的规范化，提高我国AMD的诊疗、预防和随访水平。（本文于2023年4月6日优先出版在中华眼科杂志官网）

【关键词】　黄斑变性；　诊疗准则（主题）；　循证医学

实践指南注册：国际实践指南注册与透明化平台，IPGPR‑2021CN111

Evidence‑based guidelines for diagnosis and treatment of age‑related macular degeneration in China (2023)

Chinese Vitreo‑Retina Society of Chinese Medical Association, Fundus Disease Group of Chinese Ophthalmologist Association

Corresponding author: Xu Xun, Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Eye Diseases, Shanghai

Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases,

Shanghai 200080, China, Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

【Abstract】 Age‑related macular degeneration (AMD) is the main cause of low vision and even blindness in the elderly. With the aging of our population, the number of AMD patients will continue to rise. In the past decade, the rapid development of ocular fundus imaging technology has provided a new perspective and approach for the classification, diagnosis and follow‑up of AMD. The advent of new drugs has provided more diverse intervention and treatment methods for AMD, especially for neovascular AMD, and the emphasis on accurate and individualized treatment has put forward higher requirements for retinal specialists. Therefore, based on the latest evidence‑based medical information, combined with the international guidelines and the current situation of China′s social and economic development, experts from the Chinese Vitreo‑Retinal Society of Chinese Medical Association, the Fundus Disease Group of Chinese Ophthalmologist Association, and the National Clinical Research Center for Eye Diseases gave recommendations around eight clinical problems and formed China′s guidelines for the clinical diagnosis and treatment of AMD. With the implementation of these guidelines, we can standardize the diagnosis, treatment, prevention and follow‑up of AMD in China. (This article was published ahead of print on the official website of Chinese Journal of Ophthalmology on April 6, 2023)

【Key words】

Macular degeneration;

Practice guidelines as topic;

Evidence‑based

medicine

Practice guideline registration: Practice Guideline Registration for Transparency, IPGRD‑2021CN111

组织工程中细胞与生物材料相互作用研究进展

张家盛 1,3，吴刚 2 ，邱江 1

1 中山大学附属第一医院，广东 广州 510000

2 华南理工大学 材料科学与工程学院，广东 广州 510000

3 广州华越肾科再生医学科技有限公司，广东 广州 510000

张家盛, 吴刚, 邱江. 组织工程中细胞与生物材料相互作用研究进展. 生物工程学报, 2021, 37(8): 2668-2677.

Zhang JS, Wu G, Qiu J. Interactions between cells and biomaterials in tissue engineering: a review. Chin J Biotech, 2021, 37(8):

2668-2677.

摘 要: 种子细胞、生物材料和生长因子是组织工程三要素。生物材料模拟体内细胞外基质，为细胞提供良好的生长附着环境，维持细胞的活力和功能。材料表面的理化性质和表面改性分子直接影响细胞的粘附、增殖、迁移和分化等细胞行为，进而影响细胞功能和组织再生效果。材料表面修饰分子是细胞表面粘附和生长的直接接触位置，因此细胞与生物材料表面修饰分子的相互作用是组织工程的关键。文中重点介绍表面修饰分子对细胞表型及功能的影响，为组织工程关键问题的研究提供参考。

关键词: 细胞外基质，整合素，表面修饰，机械转导，干细胞，生长因子

Interactions between cells and biomaterials in tissue engineering: a review Jiasheng Zhang1,3, Gang Wu2 , and Jiang Qiu1

1 The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong, China

2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510000, Guangdong, China

3 Asia Kidney Regenerative Medicine Technology Limited, Guangzhou 510000, Guangdong, China

Abstract: Seed cells, biomaterials and growth factors are three important aspects in tissue engineering. Biomaterials mimic extra cellular matrix in vivo, providing a sound environment for cells to grow and attach, so as to maintain cell viability and function. The physicochemical properties and modification molecules of material surface mediate cell behaviors like cell adhesion, proliferation, migration and differentiation, which in turn affect cellular function and tissue regeneration efficacy. Furthermore, the modification molecules of material surface are the direct contact point for cell adhesion and growth. Therefore, the interactions between cells and surface modification molecules are the key to tissue engineering. This review summarizes the effects of surface modification molecules on cell phenotypes and functions.

Keywords: extra cellular matrix, integrin, surface modification, mechanotransduction, stem cell, growth factor

Received: September 23, 2020;

Accepted: March 16, 2021

Supported by: Natural Science Foundation of Guangdong Province, China (No. 2016A030313190).

Corresponding author: Jiang Qiu. Tel: +86-20-87338278; E-mail: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

广东省自然科学基金 (No. 2016A030313190) 资助。