Mona Sadat Mirtaleb1,2, Mahvash Khodabandeh Shahraky1, Abdolkhaleg Deezagi3 & Danial Shafiee1

1 Department of Bioprocess Engineering, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

2 Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran. 3Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology

(NIGEB), Tehran, Iran. email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

With the advancement of biotechnology in the marine industry, an increasing utilization of marine ingredients in skincare products has been observed in recent years. Encapsulating Artemia franciscana extract and its derivatives in a novel phospholipid vesicle called hyalurosome presents innovative strategies for drug delivery systems and anti-aging products. In this study, we developed nano hyalurosomes containing Artemia franciscana active components. Partially purification of proteins in the Artemia franciscana extract was performed using ion exchange chromatography, specifically targeting Hsp40 and Artemin. The physicochemical properties of the hyalurosomes were characterized, revealing nanoparticle sizes ranging from 100 to 130 nm, zeta potential between −57 and −41.2 mV. The biological compatibility of the fabricated hyalurosomes was tested in vitro on mice fibroblast cells. Results indicated that formulations containing hyalurosomes exhibited no cytotoxicity. In-vivo studies employing H&E and Mason’s trichrome staining demonstrated an increase in the dermal layer of the skin on male mice and collagen production following treatment with different formulations containing hyalurosomes. Therefore, these formulations are considered promising candidates for anti-aging effects. Stability study at 4 °C for 60 days validated by FE-SEM imaging. In conclusion, hyalurosomes fabricated with Artemia franciscana extract and its diverse active molecules successfully achieved enhanced loading and penetration into the deeper layers of the skin, and it can be a suitable candidate for the treatment of skin aging and rejuvenation.

Keywords Nano drug delivery, Hyalurosome, Marine biotechnology, Artemia franciscana, Skin regeneration