黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
原创: 十六点五 中山二院糖尿病足中心
当各大商场又响起刘德华的“恭喜你发财、恭喜你发财”的歌声,当广州逐步走向“空城”的时候,我们知道,春节又要来到了。兄弟科室不停地在“封科”,病房几乎已经完全空掉时候,我们糖尿病足的病房满满的,换药的专科护士过得那个充实啊,就好像春节与我们无关。
经过反复权衡掂量,以及与患者、患者家属沟通,决定对于患者生长比较好的右足采取“点状植皮”,以加速患者伤口愈合,减少伤口的渗出。在进行植皮之前,首先再次对伤口进行清创,尽可能清除可能影响植皮效果的组织,之后装负压(2019年1月24日),准备下周四(2019年1月31日)在手术台上拆负压,直接植皮,之后再装负压,以促进植皮的贴敷,增加植皮的成功率。
2019年1月22日,对患者的足进行了第一次清创,效果如下:
由于各种原因,患者到中心的合作医院治疗了整整两个星期。在不断的微信沟通下,合作医院的医生进行了不懈的努力,患者继续向保足的方向又前进了几步。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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