Suradwadee Thungmungmee1 , Boonchoo Sritularak2 , Nakuntwalai Wisidsri1 , Nattakan Kanana3 , Nattika Nimmano3
1 Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand;
2 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok, Thailand;
3 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Correspondence: Nattika Nimmano, Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, Tel +66 0 2188399, Fax +66 0 2188401, Email 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Purpose: The study aimed to investigate in vitro anti-aging activities of 29 Dendrobium spp. and develop and characterize microemulsions (MEs) for topical application.
Methods: Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated.
Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2′,7′- dichlorofluorescin diacetate (DCFDA) assays, respectively. D. kentrophyllum extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.
Results: D. kentrophyllum appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and antielastase activities. The extract was safe for human skin cells at a concentration of 6.25–100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.
Conclusion: DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.
Keywords: reactive oxygen species, anti-aging activities, Dendrobium kentrophyllum, microemulsion
Magdalena Wójciak 1,* , Piotr Drozdowski 2,* , Agnieszka Skalska-Kami ´nska 1 , Martyna Zagórska-Dziok 3 , Aleksandra Ziemlewska 3 , Zofia Nizioł-Łukaszewska 3 and Małgorzata Latalska 4
1 Department of Analytical Chemistry, Medical University of Lublin, Chod´zki 4a, 20-093 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
2 Department of Plastic Surgery, Specialist Medical Centre, 57-320 Polanica-Zdrój, Poland
3 Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.Z.-D.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.Z.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Z.N.-Ł.)
4 Department of General and Pediatric Ophthalmology, Medical University of Lublin, 20-079 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.W.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (P.D.)
Citation: Wójciak, M.; Drozdowski, P.; Skalska-Kami ´nska, A.; Zagórska Dziok, M.; Ziemlewska, A.; Nizioł- Łukaszewska, Z.; Latalska, M. Protective, Anti-Inflammatory, and Anti-Aging Effects of Soy Isoflavones on Skin Cells: An Overview of In Vitro and In Vivo Studies. Molecules 2024, 29, 5790. https://doi.org/10.3390/ molecules29235790
Academic Editors: José Basilio Heredia, Luis Angel CabanillasBojórquez and Marilyn
Shomara Criollo-Mendoza
Received: 1 November 2024
Revised: 1 December 2024
Accepted: 5 December 2024
Published: 7 December 2024
Abstract: Isoflavones are found in numerous plant species within the Leguminosae family; however, soy isoflavones are particularly significant in practice and have been extensively studied in recent years. The health-promoting potential of orally administered soy isoflavones is widely documented in the scientific literature, and many review articles have been developed to highlight their significance. However, it should be noted that soy-isoflavone-rich extracts and isolated soy isoflavones, such as genistein and daidzein, are also often applied topically as ingredients in many formulations, including face creams, tonics, and emulsions. New delivery systems are continuously being developed to enhance the skin permeability of isoflavones, thus improving their efficacy. In this context, their direct activity on skin cells is an important aspect of scientific research. The anti-inflammatory, protective, and antioxidant properties of isoflavones and soy extracts make them promising cosmetic ingredients with anti-aging potential because inflammation and the accumulation of reactive oxygen species (ROS) can lead to structural and functional changes in skin cells, accelerating the aging process. This review provides an overview of research on the impact of the application of soy isoflavone extract and soy-derived isoflavones on skin cells, with a focus on the documented molecular mechanisms underlying their effects. This study aims to offer essential insights to aid in the development of functional cosmetics and future clinical applications.
Keywords: phytoestrogens; topical application; genistein; daidzein; fibroblasts; keratinocytes; skin
DF 中山二院糖尿病足中心
原创: 十六点五 中山二院糖尿病足中心
糖尿病足的切口设计是糖尿病足局部脓肿及窦道治疗的第一步,也是最关键的一步之一,由于糖尿病足脓肿和窦道的多样性,以及糖尿病足病人全身情况的不一致,因此,要找到糖尿病足切口的设计规律比较困难,我们根据大量的临床实践,制定了以下的一些原则。
在上节中提到局部鉴别皮肤组织的“死活”是一个难题,有读者问有关在创面表面用“亚甲基蓝”染料染色来鉴别的问题,这种方法是一种比较古老的方法,对于相对较干净的伤口的效果比较好,对于严重感染的伤口容易出现误报,而且为了最大程度的保护足的结构和功能,一般采取蚕食性的方法进行清创,用这些染料染色后,反而有时候会干扰对肉芽组织生长的判断,容易造成清创过多的情况,因此,目前应用较少(也比较难得到有证书能够在临床应用的这种染料)。
针对“杨氏扩散”的特殊性、沿着窦道扩散的缺点及足部手术的原则,我们设计了一种沿着窦道行纵向切开的“刘氏切法”(图1),特点是:(1)沿着窦道做平行于足纵轴切开;(2)一般在窦道开口及窦道末端切开;(3)切口长度3-4CM;(4)足弓部足底边缘部的切口非常关键。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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