The first FDA-approved drug for frostbite can save limbs from amputation, and researchers are working on “coldscreen” preventatives.

This article is excerpted from the ACS Cent. Sci. 2024, 10, 1976−1979 by Wound World.

Skin organoid transplantation promotes tissue repair with scarless in frostbite

Wenwen Wang1,‡, , Pu Liu2,3,‡, , Wendi Zhu1,‡, , Tianwei Li2, , Ying Wang1, , Yujie Wang1, , Jun Li1, , Jie Ma2,*, ,Ling Leng1,*,

1 Stem Cell and Regenerative Medicine Lab, Institute of Clinical Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China

2 State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China

3 Chongqing Key Laboratory on Big Data for Bio Intelligence, Chongqing University of Posts and Telecommunications, Chongqing 400065, China ‡ These authors contributed equally.

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J. Ma); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (L. Leng)

Abstract

Frostbite is the most common cold injury and is caused by both immediate cold-induced cell death and the gradual development of localized infammation and tissue ischemia. Delayed healing of frostbite often leads to scar formation, which not only causes psychological distress but also tends to result in the development of secondary malignant tumors. Therefore, a rapid healing method for frostbite wounds is urgently needed. Herein, we used a mouse skin model of frostbite injury to evaluate the recovery process after frostbite. Moreover, single-cell transcriptomics was used to determine the patterns of changes in monocytes, macrophages, epidermal cells, and fbroblasts during frostbite. Most importantly, human-induced pluripotent stem cell (hiPSC)-derived skin organoids combined with gelatin-hydrogel were constructed for the treatment of frostbite. The results showed that skin organoid treatment signifcantly accelerated wound healing by reducing early infammation after frostbite and increasing the proportions of epidermal stem cells. Moreover, in the later stage of wound healing, skin organoids reduced the overall proportions of fbroblasts, signifcantly reduced fbroblast-to-myofbroblast transition by regulating the integrin α5β1-FAK pathway, and remodeled the extracellular matrix (ECM) through degradation and reassembly mechanisms, facilitating the restoration of physiological ECM and reducing the abundance of ECM associated with abnormal scar formation. These results highlight the potential application of organoids for promoting the reversal of frostbite-related injury and the recovery of skin functions. This study provides a new therapeutic alternative for patients suffering from disfgurement and skin dysfunction caused by frostbite.

Keywords skin frostbite, frostbite treatment, human-induced pluripotent stem cell, skin organoids, single-cell transcriptomics

Feet Salvage Using Anterolateral Thigh Flaps after Severe Frostbite Injury: A Case Report

This article is excerpted from the  J. Pers. Med. 2024, 14, 389 by Wound World.

Development of chilblain lesions during the COVID-19 pandemic is strongly associated with cold weather: a retrospective analysis of patients from a single centre in the USA

An illustrative case of pseudo-chilblains in tropical Singapore: what is its significance?

This article is excerpted from the Singapore Medical Journal ¦ Volume 65 ¦ Supplement 1 ¦ March 2024 by Wound World.

Frostbite secondary to antimycobacterial-induced peripheral neuropathy: a case report

Shahad N Alanazi1 , Dana A Bali1 , Nawaf M Alwagdani2 , Youssof Mal3 , Maram T Alkhatieb4 , Hattan A AlJaaly1 , Zahir T Fadel1

1 Division of Plastic Surgery, Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 2Department of Orthopedic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia; 3Department of Clinical Biochemistry, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 4Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Received October 24, 2023; Accepted March 13, 2024; Epub April 15, 2024; Published April 30, 2024

Abstract: This case report describes a unique scenario in which antimycobacterial-induced peripheral neuropathy (PN) culminates in severe bilateral foot frostbite. Drug-induced peripheral neuropathy (DIPN) is explored in the context of TB treatment, highlighting the role of medications such as isoniazid (INH) and their potential to cause PN. The report highlights the importance of identifying PN in patients undergoing antimycobacterial treatment. Early recognition and proper management of PN is crucial to prevent complications. Notably, the report advocates for patient education regarding medication side effects and avoiding harmful practices, such as ice immersion, to alliviate neuropathic pain. Emphasis is directed towards the need for a multidisciplinary approach to patient care and a focus on preventative strategies to improve patient outcomes and avoid severe debilitating complications.

Keywords: Frostbite, peripheral neuropathy, isoniazid (INH), drug-induced peripheral neuropathy (DIPN)