表皮细胞去分化的表皮干细胞

赵学凯，梁自乾，张宪发，宗守凯

Mature epidermal cells dedifferentiate towards epidermal stem cells

Zhao Xue-kai, Liang Zi-qian, Zhang Xian-fa, Zong Shou-kai

Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China

Zhao Xue-kai★,Studying for master’s degree, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaoxuekai3581@ 163.com

Correspondence to: Liang Zi-qian, Professor, Master’s supervisor, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region,China Liangzqian@ yahoo.com.cn

Received: 2011-06-16 Accepted: 2011-10-10

广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市530021

赵学凯★，男，1985 年生，山东省滨州市人，汉族，广西医科大学在读硕士，主要从事创面愈合、表皮干细胞的研究。该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

通讯作者：梁自乾，教授，硕士生导师，广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

中图分类号:R318

文献标识码:B

文章编号: 1673-8225 (2011)49-09295-04

收稿日期：2011-06-16

修回日期：2011-10-10 (20110516001/WL·W)

Abstract

BACKGROUND: Epidermal stem cells as seed cells have been paid great attention on the research of wound repair and regeneration with its specific biological advantages with the development of molecular biology and bioengineering.

OBJECTIVE: To review the recent advances in the mature epidermal cells dedifferentiating towards epidermal stem cells.

METHODS: The first author retrieved PubMed database, Highwire database (Highwire.standford.edu) and CNKI for articles published from January 1998 to January 2011. The key words were “dedifferentiation, epidermal stem cell, epidermal cell, induction” in English and Chinese. A total of 2 754 literatures were collected, and finally 46 papers were included in result analysis.

RESULTS AND CONCLUSION: The dedifferentiated epidermal stem cells have some characteristics of native epidermal stem cells and perhaps they may act as a new vehicle for wound repair and regeneration. Both of the native epithelial stem cells and the dedifferentiated epithelial stem cells can promote the wound repair and their effects were on the same level. Because differentiation from epidermal cells into epidermal stem cells has many questions, so far, there are many animal researches, but its extensive clinical application deserves further development.

Zhao XK, Liang ZQ, Zhang XF, Zong SK. Mature epidermal cells dedifferentiate towards epidermal stem cells.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(49):9295-9298. [http://www.crter.cn http://en.zglckf.com]

摘要

背景：表皮干细胞以显著的自我更新和多向分化潜能及皮肤组织工程学中诱人的应用前景，作为“种子细胞”成为皮肤创伤研究领域的热点之一。

目的：综述表皮细胞去分化逆转为表皮干细胞的研究近况。

方法：应用计算机检索 1998-01/2011-01 PubMed、Highwire 和中国期刊全文数据库相关文章，检索词“dedifferentiation，epidermal cells，epidermal stem cells，induction，去分化，表皮细胞，表皮干细胞，诱导”。共检索到文献 2 754 篇，最终纳入符合标准的文献 46 篇。

结果与结论：去分化来源的表皮干细胞与机体自身来源的表皮干细胞之间在形态学和功能有许多相似点，并可表达表皮干细胞的特异性标记物。表皮细胞去分化途径制造表皮干细胞，可以作为机体自身来源表皮干细胞的替代细胞应用于皮肤损伤重建和再生的相关研究，为实现皮肤创伤由解剖修复到功能修复的“完美修复”提供新思路。

关键词：表皮细胞；去分化；表皮干细胞；诱导；皮肤组织工程；创面愈合；综述文献

doi:10.3969/j.issn.1673-8225.2011.49.041

赵学凯，梁自乾，张宪发，宗守凯．表皮细胞去分化的表皮干细胞[J].中国组织工程研究与临床康复，2011，15(49):9295-9298.

[http://www.crter.org http://cn.zglckf.com]

表皮干细胞——细胞疗法的新希望

徐垄，柴琛，滕永军

兰州大学第一医院普外科，甘肃兰州730000

摘要：对表皮干细胞的特征、亚群、调控通路、微环境、与皮肤肿瘤的关系及临床应用作一综述，总结近年来表皮干细胞的最新研究进展，为进一步研究提供思路和研究方向。

关键词：表皮干细胞；增殖；分化；信号通路；皮肤肿瘤，临床应用

中图分类号：R6

文献标识码：A

doi：10．13885／j．issn．1000—2812．2017．01．013

收稿日期：2016—12-03

基金项目：甘肃省卫生行业科研计划项目(GSWST2011-05)

作者简介：柴琛，博士，主任医师，研究方向为创伤愈合，e-mail：chasechai@126．com，通信联系人

滕永军，博士，主任医师，研究方向为创伤愈合，e—mail：Tengyj@lan．edu．cn，共同通信联系人

Epidernal stem cell：a new hope for cell therapy

Xu Kun，Chai Chen，Teng Yong-jun

Department ofGeneral Surgery,First Hospital ofLanzhou University,Lanzhou 730000，China

Abstract：In this paper,we reviewed the characteristics，microenvironment，subpopulation，regulatory path—ways，clinical applications of epidermal stem cells(ESCs)and the relationship between skin cancer and ESCs，and summarized recent advances in ESCs to provide some ideas andresearch directions for relevant researchers．

Keywords：epidermal stem cells；proliferation；differentiation；signaling pathway；skin cancer；clinical application

表皮干细胞生物学特性研究现状

蓝蔚，刘德伍，毛远桂

南昌大学第一附属医院烧伤中心，江西省南昌市330006

蓝蔚★，男，1979年生，江西省大余县人，畲族，南昌大擘在读硕士，主要从事烧伤创面修复与皮肤组织工程方面的研究。lan_wei@163．Com

通讯作者：刘德伍，博士，主任医师，博士生导师，南昌大学第一附属医院烧伤中心，江西省南昌市330006dewuliu@126．com

国家自然科学基金(30560058)*：江西省科技厅重大科技招标项目(200604)*：江西省教育厅科研项目(赣教技字『2005】187)+；江西省卫生厅科研项目(20041038)*

中图分类号：R394．2文献标识码：A 文章编号：1673．8225 (2008)25．049364)5

收稿日期：20cr7．10．19 修回日期：2008-02．28 (07-50-10-5643/ZS●Y)

Centre of Bums，the First Affiliated Hospital of Nanchang University,Nanchang 330006． Jiangxi Province，China

Lan Wei★．Smdyiag for ma．qter’s degree， Centre of Burns，the First Amhated Hospital of Nanchang University,Nanchang 330006， Jiangxi Province。China Lan wei@163．Com

Correspondelice to： Liu De-wu，Doctor, Chief physician， Tutor of doctor, Centre of Burns，the First Affiliated Hospital of Nanchang University,Nanehang 330006，liangxi Province．Cbina dewuliu@126．tom

Supported by：the National Natural Science Foundation of China．No．30560058+：the Great Invited Tenders Foundation of Jiangxi Provincial Science and Technology Bureau． No．200604*：the Science Research Foundation of Jiangxi Provincial Education Bureau， No 2005一187+：the Science Research Foundation of Jiangxi Provincial Health Bureau，No， 20041038*

Received：2007．．10．．19 Accepted：2008-02-28

Abstract：Epidermal stem cell is a specific stem cell．which locates in epidermal basaI layer and the eminence of hair follicle．Epidermal stem cells have the characteristic of the undifferentiated cells in the morphology．and are tile biological source of the skin and its subsidiary in the development．repair and reconstruction．At present．the identification markers of epidermal stem cell include integrin，keratin．proliferating cell nuclear antigen，genic material and connectin．The proliferation and differentiation of epidermal stem cell arc influenced by many factors．such as integrin．signal transduction pathway and cytokines．Current researches of epidermal stem cell in skin wound healing，building of tissue．engineered skin，gene therapy and other areas have made great progress，but there are still many problems to wait for solving，such as establishing an absolutely recognized specific marker，the directed differentiation from embryonic stem cells to epidermal stem cells．how to mainrain its stem cell characteristics and induce large amplification．whether it will have a malignant transformation in clinical application．

Lan，W. Lu DW，Mao YG．Biological characteristics of epidermal stem cells nerves．Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kaugfu 2008；12(25)：4936-4940(China) [wWW．zglckf．com／zglckf／ejoumal／upfiles／08—25／25k一4936(ps)．pdf]

摘要：表皮干细胞是来源于胚胎外胚层皮肤组织的专能干细胞，丰要分布于表皮基底层和毛囊隆突部，形态学上具有未分化细胞的特征，在生物学方面是皮肤及其附属器发牛、修复、改建的源泉。现阶段对表皮T细胞的鉴定需采用整合素、角蛋白、增殖细胞核抗原、基因物质及连接蛋白等多个标记物，其增殖分化受整合素、信弓转导通路及细胞冈子等冈素影响。

目前表皮干细胞研究在皮肤创面修复、组织工程皮肤的构建、基因治疗等方面均L二取得显著进展，但仍存在如何确立一种绝对公认的特异标记物、从胚胎T-细胞向表皮干细胞分化培养过程中怎样维持其干细胞特性以及快速大量扩增、应用于临床是否会恶性变而产生医疗安全等急需解决的问题。

关键词：表皮干细胞；定位：标记物；增殖分化；综述文献

蓝蔚，刘德伍，毛远桂．表皮干细胞生物学特性研究现状．[J】中国组织工程研究与临床康复，2008，12(25)：4936—4940

【www．zglckf．conffzglckf／ejournal／upfiles／08—25／25k一4936(ps)．pdf]

氨基己酸激活人表皮干细胞中Oct4基因的表达

刘惠玲1，伍明俊2 9陈关霞1，李美蓉1，伍志强1，赵亚力1，韩为东1，孙晓艳1

1解放军总医院基础医学所，北京100853；2中国医学科学院输血研究所血液免疫研究中心，成都 610081

摘要：目的摸索并建立表皮干细胞Oct4基因的非转基因诱导方法。方法采用RT—PCR方法，检测经6一氨基己酸刺激48h后表皮干细胞Oet4基因的转录水平。结果“氨基己酸能有效激发表皮干细胞Oct4基因的转录。结论RT—PCR方法检测表皮干细胞Oct4基因转录水平变化的准确度高、重复性好，实验步骤简单，是初步筛选诱导基凶转录表达小分子化合物的实用方法。

关键词：6一氨基己酸；表皮干细胞；Oct4

中图分类号：R 644

文献标识码：A

文章编号：1005一l 139(201 1)01-0070-03

收稿日期：2010—04—20

修回日期：2010-05—06

基金项目：国家自然科学基金项目(30730090，30901564)；北京市科技新星计划资助项目(2008853)Supported by the National Natural Science Foundation of China(30730090，30901 564)

作者简介：刘惠玲，女，本科，实验师。Email：liu hui_ling垣黼u．㈣

6-aminohexanoic acid activates expression of Oct4 gene in human epidermic stem cells LIU Hui—lin91。WU Ming-jun2，CHEN Mei．xial，LI Mei—ron91，WU Zhi·qian91，ZHAO Ya—lil，HAN Wei．don91，SUN Xiao-yanl 1Institute of Basic Medical Science，Chinese PLAGeneral Hospital，Beijing 100853，China；2Research Center of Blood Immunology， Institute of Blood Transfusion，Chinese Academy ofMedical Sciences，Chengdu 610081，Sichuan Province，China The first author：LIU Hui-ling．Emall：liu_hui—ling@sohu．com

Abstract：Objective To develop the non—transgenic method for inducing transcription of oct4 gene in epidermic stem cells．

Methods Rr-PCR was performed to measure the transcription level of oct4 gene in epidermic stem cells 48h after activated by 6．aminohexanoic acid．Results 6．aminohexanoic acid effectively activated the transcription of OCt4 in epidermic stem ceils．

Conclusion RT-PCR Can accurately and repeatedly detect the transcription level of oct4 gene in epidermic stem ceils．and iS thUS a simple and practical method for screening the potential small molecular compounds expressed in inducing transcription genes．

Key words：6．Aminohexanoic Acid；Epidermal Stem Cells；Oct4

基于PINK1/Parkin通路介导的线粒体自噬 探讨中药丹黄散对糖尿病溃疡的促愈机制

王庚新 1 ，张春玲 2* ，赵 伟 2 ，陈 露 2 ，邸铁涛 2 ，张 云 1 ，王艳辉 1 ，刘海艳 1

1. 贵州中医药大学，贵州 550002；
2. 贵州中医药大学第二附属医院

基金项目 国家自然科学基金资助项目，编号：81960805；贵州省研究生教育创新计划项目，编号：黔教合 YJSCXJH(2020)161

作者简介 王庚新，硕士研究生在读

人表皮角质形成细胞急性光损伤模型的构建

邓蕙妍 肖峰 高爱莉 李润祥 朱慧兰 万苗坚

DOI：10．3969／g．issn．0253-9802．2013．08．012

基金项目：广东省自然科学基金($2011010005956)，广州市医药卫生科技项目(20131A011130)

作者单位：510095广州，广州市皮肤病防治所(邓蕙妍，高爱莉。李润祥，朱慧兰)；510630广州，中山大学附属第三医院(肖峰，万苗坚)

通讯作者。朱慧兰，E．mail：zhlhuilan@hotmail．com；万苗坚，E-mail：w13725313465@163．oom

【摘要】

目的探讨长波紫外线(UVA)和中波紫外线(UVB)对人永生化角质形成细胞(HaCaT细胞)造成急性损伤所需要的最小剂量，为构建人表皮细胞急性光损伤模型提供科学依据。方法用不同剂量的UVA或UVB照射HaCaT细胞。观察细胞形态学改变，检测细胞增殖活性(吸光度)和凋亡率。结果2．5—10 J／era2UVA照射对HaCaT细胞无损伤，20～40 J／cm2可致细胞出现形态学改变，与空白对照组相比增殖活性降低，凋亡率增加(P<0．01)；11．4～34．2 mJ／cm2UVB照射对HaCaT细胞无损伤，随照射剂量增加，细胞出现不同程度损伤，与空白对照组相比增殖活性降低，凋亡率增加(P<0．01)。结论UVA和UVB照射均可构造急性光损伤模型。HaCaT细胞对UVB敏感性更高，引起急性光损伤所需的最小剂量更低。

【关键词】

长波紫外线；中波紫外线；角质形成细胞；光损伤

Construction of acute light damage model with human epidermal keratinocytes

DENG Hui—yan．XIAO

Feng，GAO Ai—li．LI Run—xiang，ZHU Hui—lan，WAN Miao-jia,L Guangzhou Dermatosis Preventing＆Curing

Institute，Guangzhou 510095，China

Corresponding

author：ZHU

Hui—lan，E—mail：zhlhuilan@hotmaiL COrn；WAN Miao-jian，E—mail：

w13725313465@163．corn

【Abstract】

Objective

To explore a minimum dose of ultraviolet A(UVA)and ultraviolet B(UVB) that could cause immortalized keratinocytes(HaCaT cells)acute injury，and provide a scientific basis for building human epidermal cell acute light damage model．

Methods HaCaT cells were treated with different dose of UVA／UVB．The morphological changes，as well as proliferation activity and apoptosis rate were ob— served．

Result No injury changes were onservd with radiating dose of low radiation dose as 2．5—1 0 J／cm2 UVA．Yet 20-40 J／cm2 caused cellular morphological changes，with decreased proliferative activity and in—creased apoptosis rate(P<0．01)．No injury changes were observed with radiating dose of 1 1．4～34．2 mJ／ cm2 UVB．However，ceils manifested with varied morphological changes，activity and apoptosis rate with te in— vrease of UVB dosage(P<0．01)．

Conclusion Both UVA and UVB radiation can be used as construction to make acute light damage model．HaCaT ceils presented with more sensitivity with UVB，showing lower mini—mum required dose to cause acute light damage．As a dose-dependent model，type and degree of radiations were alternative in different experimental settings．

【Key words】 UVA；UVB；HaCaT cells；Photodamage