A Comprehensive Review of Stem Cell Conditioned Media Role for Anti-Aging on Skin

yatulloh Alquraisy1 , Gofarana Wilar 2 , Ahmed Fouad Abdelwahab Mohammed3 , Ali El-Rayyes4 , Cecep Suhandi 1, Nasrul Wathoni 1

1  Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Padjadjaran, Sumedang, 45363, Indonesia;

2  Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Padjadjaran, Sumedang, 45363, Indonesia; 3 Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt; 4 Department of Chemistry, College of Science, Northern Border University, Arar, Saudi Arabia

Correspondence: Nasrul Wathoni, Email 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Abstract: Various studies have been widely conducted on conditioned medium for the development of anti-aging preparations, including the utilization of stem cells, which present a promising alternative solution. This narrative review aims to understand the latest developments in various conditioned medium stem cell applications for anti-aging on the skin. A search of the Scopus database yielded publications of interest. The research focused on articles published without restrictions on the year. After finding 68 articles in the search results, they moved on to the checking phase. Upon comprehensive literature review, 23 articles met the inclusion criteria, while 45 articles were deemed ineligible for participation in this research. The results of the review indicate that conditioned medium from various stem cells has demonstrated success in reducing risk factors for skin aging, as proven in various tests. The successful reduction of the risk of skin aging has been established in vitro, in vivo, and in clinical trials. Given the numerous studies on the progress of exploring and utilizing conditioned medium, it is expected to provide a solution to the problem of skin aging.

Keywords: conditioned medium, stem cell, anti-aging, skin aging

Oral Administration of Nacre Extract from Pearl Oyster Shells Has Anti-Aging Effects on Skin and Muscle, and Extends the Lifespan in SAMP8 Mice

Hana Yamamoto, Nanami Shimomura and Yasushi Hasegawa

College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.Y.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (N.S.)

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。; Tel.: +81-143-46-5745

Abstract: Pearl oysters have been extensively utilized in pearl production; however, most pearl oyster shells are discarded as industrial waste. In a previous study, we demonstrated that the intraperitoneal administration of pearl oyster shell-derived nacre extract (NE) prevented d-galactose-induced brain and skin aging. In this study, we examined the anti-aging effects of orally administered NE in senescence-accelerated mice (SAMP8). Feeding SAMP8 mice NE prevented the development of aging-related characteristics, such as coarse and dull hair, which are commonly observed in aged mice. Additionally, the NE mitigated muscle aging in SAMP8 mice, such as a decline in grip strength. Histological analysis of skeletal muscle revealed that the NE suppressed the expression of aging markers, cyclin-dependent kinase inhibitor 2A (p16) and cyclin-dependent kinase inhibitor 1 (p21), and increased the expression of sirtuin1 and peroxisome proliferator-activated receptor gamma coactivator 1 (PGC1)- α, which are involved in muscle synthesis. These findings suggest that the oral administration of NE suppresses skeletal muscle aging. Moreover, NE administration suppressed skin aging, including a decline in water content. Interestingly, oral administration of NE significantly extended the lifespan of SAMP8 mice, suggesting that its effectiveness as an anti-aging agent of various tissues including skeletal muscle, skin, and adipose tissue.

Keywords: skeletal muscle aging; nacre extract; lifespan extension; sarcopenia; skin aging

Citation: Yamamoto, H.; Shimomura,

N.; Hasegawa, Y. Oral Administration

of Nacre Extract from Pearl Oyster

Shells Has Anti-Aging Effects on Skin

and Muscle, and Extends the Lifespan

in SAMP8 Mice. Pharmaceuticals 2024,

17, 713. https://doi.org/

10.3390/ph17060713

Academic Editors: Mariia Shanaida,

Geir Bjørklund and Olha

Mykhailenko

Received: 16 May 2024

Revised: 28 May 2024

Accepted: 29 May 2024

Published: 31 May 2024

Macrofungal Extracts as a Source of Bioactive Compounds for Cosmetical Anti-Aging Therapy: A Comprehensive Review（2-2）

This article is excerpted from the Nutrients 2024, 16, 2810 by Wound World.

Macrofungal Extracts as a Source of Bioactive Compounds for Cosmetical Anti-Aging Therapy: A Comprehensive Review（2-1）

Maja Paterska 1 , Bogusław Czerny 2,3 and Judyta Cielecka-Piontek 4,*

1 Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

2 Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, 62-064 Plewiska, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

3 Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University in Szczecin, 71-210 Szczecin, Poland

4 Department of Pharmacognosy and Biomaterials, Faculty of Pharmacy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Citation: Paterska, M.; Czerny, B.;

Cielecka-Piontek, J. Macrofungal

Extracts as a Source of Bioactive

Compounds for Cosmetical

Anti-Aging Therapy: A

Comprehensive Review. Nutrients

2024, 16, 2810. https://doi.org/

10.3390/nu16162810

Academic Editors: Arrigo Cicero and

Akiko Kojima-Yuasa

Received: 26 June 2024

Revised: 21 August 2024

Accepted: 22 August 2024

Published: 22 August 2024

Abstract: For centuries, mushrooms have been used as a component of skincare formulations. Environmental stresses and a modern lifestyle expose the skin to accelerated aging. To slow down this process, natural anti-aging skincare ingredients are being sought. In this review, 52 scientific publications about the effects of chemical compounds extracted from the fruiting bodies of macrofungi on skin cells were selected. The effects of extracts from nine species that are tested for anti-aging effects have been described. According to available literature data, macrofungi contain many polysaccharides, phenolic compounds, polysaccharide peptides, free amino acids, sterols, proteins, glycosides, triterpenes, alkaloids, which can have an anti-aging effect on the skin by acting as antioxidants, photoprotective, skin whitening, moisturizing, anti-inflammatory and stabilizing collagen, elastin and hyaluronic acid levels in the skin.

Keywords: anti-aging; Trametes versicolor; Schizophyllum commune; Tremella fuciformis; Pleurotus ostreatus; Agaricus subrufescens; Volvariella volvacea; Ganoderma lucidum; Letinula edodes; Inonotus obliquus; mushrooms; macrofungi

Exploring Anti-Aging Potential of Dendrobium Species and Novel Microemulsion Delivery of Dendrobium kentrophyllum Extract for Anti-Aging Effect

Suradwadee Thungmungmee1 , Boonchoo Sritularak2 , Nakuntwalai Wisidsri1 , Nattakan Kanana3 , Nattika Nimmano3

1 Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand;

2 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok, Thailand;

3 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand

Correspondence: Nattika Nimmano, Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, Tel +66 0 2188399, Fax +66 0 2188401, Email 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Purpose: The study aimed to investigate in vitro anti-aging activities of 29 Dendrobium spp. and develop and characterize microemulsions (MEs) for topical application.

Methods: Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated.

Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2′,7′- dichlorofluorescin diacetate (DCFDA) assays, respectively. D. kentrophyllum extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.

Results: D. kentrophyllum appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and antielastase activities. The extract was safe for human skin cells at a concentration of 6.25–100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.

Conclusion: DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.

Keywords: reactive oxygen species, anti-aging activities, Dendrobium kentrophyllum, microemulsion

Protective, Anti-Inflammatory, and Anti-Aging Effects of Soy Isoflavones on Skin Cells: An Overview of In Vitro and In Vivo Studies

Magdalena Wójciak 1,* , Piotr Drozdowski 2,* , Agnieszka Skalska-Kami ´nska 1 , Martyna Zagórska-Dziok 3 , Aleksandra Ziemlewska 3 , Zofia Nizioł-Łukaszewska 3 and Małgorzata Latalska 4

1 Department of Analytical Chemistry, Medical University of Lublin, Chod´zki 4a, 20-093 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

2 Department of Plastic Surgery, Specialist Medical Centre, 57-320 Polanica-Zdrój, Poland

3 Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.Z.-D.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.Z.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Z.N.-Ł.)

4 Department of General and Pediatric Ophthalmology, Medical University of Lublin, 20-079 Lublin, Poland; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.W.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (P.D.)

Citation: Wójciak, M.; Drozdowski, P.; Skalska-Kami ´nska, A.; Zagórska Dziok, M.; Ziemlewska, A.; Nizioł- Łukaszewska, Z.; Latalska, M. Protective, Anti-Inflammatory, and Anti-Aging Effects of Soy Isoflavones on Skin Cells: An Overview of In Vitro and In Vivo Studies. Molecules 2024, 29, 5790. https://doi.org/10.3390/ molecules29235790

Academic Editors: José Basilio Heredia, Luis Angel CabanillasBojórquez and Marilyn

Shomara Criollo-Mendoza

Received: 1 November 2024

Revised: 1 December 2024

Accepted: 5 December 2024

Published: 7 December 2024

Abstract: Isoflavones are found in numerous plant species within the Leguminosae family; however, soy isoflavones are particularly significant in practice and have been extensively studied in recent years. The health-promoting potential of orally administered soy isoflavones is widely documented in the scientific literature, and many review articles have been developed to highlight their significance. However, it should be noted that soy-isoflavone-rich extracts and isolated soy isoflavones, such as genistein and daidzein, are also often applied topically as ingredients in many formulations, including face creams, tonics, and emulsions. New delivery systems are continuously being developed to enhance the skin permeability of isoflavones, thus improving their efficacy. In this context, their direct activity on skin cells is an important aspect of scientific research. The anti-inflammatory, protective, and antioxidant properties of isoflavones and soy extracts make them promising cosmetic ingredients with anti-aging potential because inflammation and the accumulation of reactive oxygen species (ROS) can lead to structural and functional changes in skin cells, accelerating the aging process. This review provides an overview of research on the impact of the application of soy isoflavone extract and soy-derived isoflavones on skin cells, with a focus on the documented molecular mechanisms underlying their effects. This study aims to offer essential insights to aid in the development of functional cosmetics and future clinical applications.

Keywords: phytoestrogens; topical application; genistein; daidzein; fibroblasts; keratinocytes; skin