伤口世界

星期五, 17 4月 2026

Beyond the genome: protecting the proteome may be the key to preventing skin aging

Brigitte DRÉNO1 Isabelle BENOIT2 Eric PERRIER2 Miroslav RADMAN3

1 INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy, INCIT, UMR 1302/EMR6001, Nantes Université, Nantes, France

2 NAOS-ILS, Aix-en-Provence, France,

3 Mediterranean Institute for Life Sciences, Split, Croatia, Naos Institute for Life Sciences, Aix-en-Provence, France, Université R.-Descartes Paris-5, Faculté de Médecine, INSERM U1, Paris, France

Reprints: Brigitte Dréno<该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。> <该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。>

Isabelle Benoit <该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。>

Eric Perrier <该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。>

Skin aging is associated with a progressive decline in physiological functions, skin cancers and, ultimately, death. It may be categorized as intrinsic or extrinsic, whereby intrinsic aging is attributed to chrono-logical and genetic factors. At the molecular level, skin aging involves changes in protein conformation and function. The skin proteome changes constantly, mainly through carbonylation; an irreversible phenomenon leading to protein accumulation as toxic aggregates that impair cellular physiology and accelerate skin aging. This review details the central role of proteostasis during skin aging and why proteome protection may be a promising approach in mitigating skin aging. A comprehensive literature review of 87 articles focusing on the proteome, proteostasis, proteotoxicity, protein carbonylation, and the impact of the damaged proteome on aging, and in particular skin aging, was conducted. Skin aging is associated with deficiencies in the repair mechanisms of DNA, transcriptional control, mitochondrial function, cell cycle control, apoptosis, cellular metabolism, changes in hormonal levels secondary to toxicity of damaged proteins, and cell-to-cell communication for tissue homeostasis, which are largely controlled by proteins. In this context, a damaged proteome that leads to the loss of proteostasis may be considered as the first step in tissue aging. There is growing evidence that a healthy proteome plays a central role in skin and in maintaining healthy tissues, thus slow-ing down the process of skin aging. Hence, protecting the proteome against oxidative or other damage may be an appropriate strategy to prevent and delay skin aging.

Keywords: proteome, proteostasis, protein carbonylation, skin aging

星期四, 16 4月 2026

Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

Beatrice Dyring-Andersen1,2,3,4, Marianne Bengtson Løvendorf5, Fabian Coscia1 , Alberto Santos1 , Line Bruun Pilgaard Møller1 , Ana R. Colaço1 , Lili Niu1 , Michael Bzorek6, Sophia Doll7, Jørgen Lock Andersen8, Rachael A. Clark2, Lone Skov 4, Marcel B. M. Teunissen 9 & Matthias Mann 1,7✉

Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative pro-teomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research (https://skin.science/).

1Novo Nordisk Foundation (NNF) Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. 2Department of Dermatology, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA. 3 Leo Foundation Skin Immunology Research Center, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. 4Department of Dermatology and Allergology, Herlev and Gentofte Hospital, University of Copenhagen, Hellerup, Denmark. 5 Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark. 6Department of Surgical Pathology, Zealand University Hospital, Næstved, Denmark. 7Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. 8Department of Plastic and Breast Surgery, Zealand University Hospital, Roskilde, Denmark. 9Department of Dermatology, Amsterdam University Medical Centers, location AMC, Amsterdam, Netherlands. ✉email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

星期三, 15 4月 2026

Quantitative proteomics analysis of young and elderly skin with DIA mass spectrometry reveals new skin aging-related proteins

Jing Ma1,2, Mengting Liu1,2, Yaochi Wang1,2, Cong Xin1,2, Hui Zhang1,2, Shirui Chen1,2, Xiaodong Zheng1,2, Xuejun Zhang1,2, Fengli Xiao1,2,3 , Sen Yang1,2

1Department of Dermatology of First Affiliated Hospital, and Institute of Dermatology, Anhui Medical University, Hefei, Anhui, China

2 Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui, China

3 The Center for Scientific Research of Anhui Medical University, Hefei, Anhui, China

Correspondence to: Sen Yang, Fengli Xiao;

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Keywords: aging, epidermal proteins, skin rejuvenation and aging, proteome, mass spectrometer

Received: February 25, 2020

Accepted: May 27, 2020

Published:

Copyright: Ma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

Skin aging is a specific manifestation of the physiological aging process that occurs in virtually all organisms In this study, we used data independent acquisition mass spectrometry to perform a comparative analysis of protein expression in volar forearm skin samples from of 20 healthy young and elderly Chinese individuals. Our quantitative proteomic analysis identified a total of 95 differentially expressed proteins (DEPs) in aged skin compared to young skin. Enrichment analyses of these DEPs (57 upregulated and 38 downregulated proteins) based on the GO, KEGG, and KOG databases revealed functional clusters associated with immunity and inflammation, oxidative stress, biosynthesis and metabolism, proteases, cell proliferation, cell differentiation, and apoptosis. We also found that GAPDH, which was downregulated in aged skin samples, was the top hub gene in a protein-protein interaction network analysis. Some of the DEPs identified herein had been previously correlated with aging of the skin and other organs, while others may represent novel age-related entities. Our non-invasive proteomics analysis of human epidermal proteins may guide future research on skin aging to help develop treatments for age-related skin conditions and rejuvenation.

星期二, 14 4月 2026

Inflammasomes as therapeutic targets in human diseases

Yangxin Li1 , Hui Huang 2, Bin Liu3 , Yu Zhang1 , Xiangbin Pan4 , Xi-Yong Yu5 , Zhenya Shen1 and Yao-Hua Song6

Inflammasomes are protein complexes of the innate immune system that initiate inflammation in response to either exogenous pathogens or endogenous danger signals. Inflammasome multiprotein complexes are composed of three parts: a sensor protein, an adaptor, and pro-caspase-1. Activation of the inflammasome leads to the activation of caspase-1, which cleaves pro-inflammatory cytokines such as IL-1β and IL-18, leading to pyroptosis. Effectors of the inflammasome not only provide protection against infectious pathogens, but also mediate control over sterile insults. Aberrant inflammasome signaling has been implicated in the development of cardiovascular and metabolic diseases, cancer, and neurodegenerative disorders. Here, we review the role of the inflammasome as a double-edged sword in various diseases, and the outcomes can be either good or bad depending on the disease, as well as the genetic background. We highlight inflammasome memory and the two-shot activation process. We also propose the M- and N-type inflammation model, and discuss how the inflammasome pathway may be targeted for the development of novel therapy. Signal Transduction and Targeted Therapy (2021) 6:247 ; https://doi.org/10.1038/s41392-021-00650-z

1Institute for Cardiovascular Science and Department of Cardiovascular Surgery, First Affiliated Hospital and Medical College of Soochow University, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, Jiangsu, P. R. China; 2 Cardiovascular Department, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, P. R. China; 3 Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, P. R. China; 4 Department of Cardiac Surgery, Fuwai Hospital, Beijing, P. R. China; 5 Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, Guangdong, P. R. China and 6 Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Soochow University, National Clinical Research Center for Hematologic Diseases, the First Affiliated Hospital of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China Correspondence: Yangxin Li (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。) or Zhenya Shen (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。) or Yao-Hua Song (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。) These authors contributed equally: Yangxin Li, Hui Huang, Bin Liu.

星期一, 13 4月 2026

原发性肝癌诊疗指南（2026 年版）

名誉主任委员：汤钊猷郑树森王学浩陈孝平董家鸿窦科峰滕皋军夏强蔡秀军

主任委员： 樊嘉

执行主任委员：周俭

副主任委员： 秦叔逵沈锋蔡建强李强王伟林陈敏山孙惠川向华吴泓

介入及局部治疗学组组长： 滕皋军

副组长：韩国宏颜志平王茂强刘瑞宝郭金和曾昭冲梁萍王忠敏

内科及系统治疗组组长：秦叔逵

副组长：任正刚侯金林张艳桥刘秀峰潘宏铭毕锋

外科学组组长：周俭

副组长：周伟平刘连新文天夫匡铭张必翔陶开山

影像学组组长：曾蒙苏

副组长：梁长虹陈敏严福华徐辉雄谢晓燕居胜红

病理学组组长：丛文铭

副组长：纪元云径平李增山

委员（按姓氏拼音排序）：

白雪莉蔡定芳陈卫霞陈亚进陈拥军成文武程树群代智戴朝六董辉杜世锁高强郭荣平郭文治郭玮郭亚兵花宝金黄晓武蒋涵羽荚卫东李家平李秋李涛李相成李汛李亚明李晔雄梁军梁霄凌昌全刘辉刘嵘刘天舒卢实春吕国悦麻勇毛一雷孟志强彭涛任伟新饶圣祥施国明石洪成石明史颖弘宋天强谭广王建华王葵王鲁王鹏王文涛王晓颖王征王志明向邦德夏君肖永胜邢宝才徐建明徐钧杨建勇杨欣荣杨业发杨云柯姚小红尹震宇袁振刚曾红梅曾永毅曾勇张博恒张雷达张水军张倜张志伟赵明赵永福郑红刚周乐杜朱继业朱康顺

秘书长： 孙惠川

副秘书长：王征史颖弘刘嵘张岚

秘书组: 肖永胜杨春吴志峰丁振斌朱小东周正君黄晓勇韩红朱海东仲斌演张巍

星期六, 11 4月 2026

Current strategies and priorities for diabetic footwear design and production: a cross-European exploratory survey of clinicians and shoemakers

Hadi Sarlak1,2 · Kamran Shakir1,2 · Giulia Rogati2 · Alberto Leardini2 · Lisa Berti1,3 · Paolo Caravaggi2

Abstract

Background Therapeutic footwear plays an important role in preventing ulceration in people with diabetes. Despite guide-lines recommending offloading footwear for individuals at risk of ulceration, limited data are available on the alignment between current industrial practices, clinical expectations, and evidence. This study explored current practices, priorities and challenges associated with diabetic footwear from manufacturers’ and clinicians’ perspectives across Europe.

Methods An exploratory cross-sectional survey was conducted between May and October 2025 to gather insights from diabetic footwear manufacturers and clinicians involved in diabetic foot care across Europe. A 26-item questionnaire was developed to explore product design, materials, innovation, and adherence to guidelines among manufacturers. Additionally, a separate 14-item questionnaire for clinicians examined footwear prescription, patient barriers, and industry communica-tion. Quantitative data were analysed descriptively, and open-text responses underwent thematic analysis.

Results Nine manufacturers and twelve clinicians participated in the survey. While only half of the Manufacturers that completed the survey reported having a research and development department, most reported adopting data-driven design approaches (n=8) and scientific literature (n=7). Offloading, internal volume, and toe protection were the highest-ranking priorities for manufacturers, whereas aesthetics ranked lowest. Clinicians, conversely, prioritised accommodation of defor-mities, offloading, and comfort, and highlighted poor aesthetics and shoe weight as major limitations to adherence.

Conclusions This exploratory study suggests partial alignment between diabetic footwear manufacturers and clinicians on functional and offloading features but highlights gaps in aesthetics, materials, and sustainability. Better collaboration, data-driven innovation, and clearer product specifications could improve user adherence, prescription efficacy, and the preventive role of therapeutic footwear in diabetes care.

Keywords Diabetic foot ulcers · Therapeutic footwear · Offloading · Survey · Footwear design

Communicated by Massimo Federici.

Kamran Shakir