李孝建

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26 8月 2019

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Author : 伤口世界

专长：重症烧伤救治。广东省医学会烧伤科分会主任委员、广东省医疗行业协会伤口管理分会副主委。

广东红十字会医院烧伤整形科主任医师，博士研究生导师。烧伤科大主任、烧伤ICU主任。

Latest from 伤口世界

Bactericidal and anti-inflammatory activity of defined hypochlorite solution
Periprocedural Use of Hypochlorous Acid Mist for Improving Healing and Cosmesis of the Face After Laser
Effect of Sodium Hypochlorite 0.05% on MMP-9 Extracellular Release in Chronic Wounds
Hypochlorous Acid for Wound Healing in Diabetic Rats: Effect on MMP-9 and Histology
Topical Hypochlorous Acid (HOCl) as a Potential Treatment of Pruritus

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Bactericidal and anti-inflammatory activity of defined hypochlorite solution 2026-11-09 00:00

Christopher David Chapman ^1,2☯*, Myles Hyla Edward Dakin ², Richard Aspinall^1☯

¹Centre for Intelligent Healthcare, Coventry University, United Kingdom, ²Hypo-stream Limited, Cambridge, United Kingdom

☯ These authors contributed equally to this work.

Abstract

Background

Hypochlorite is an antiseptic that has been used for over a century for industrial sterilisation and antisepsis. More recently, the topical application of hypochlorite to severe burn patients increased their rate of survival post infection with Gram-negative bacteria, of which antisepsis cannot be solely responsible.

Methodology

Broth microdilution experiments were performed to confirm anti-microbial activity. Cytokine exposure was performed by dialytic exposure of serum spiked with either interleukin-6 or interleukin-10, accounting for contextually appropriate organic load. Cytokine degradation was measured by immunoassay and by functional bioassay utilising HEK-Blue cells. Hypochlorite mediated cytotoxicity against dermal cells was assessed via direct exposure, followed by a 1-hour attenuation period. Resazurin was utilised to assay the impact of hypochlorite on cell viability.

Results

We have shown that this formulation of hypochlorite in isotonic saline has multiple mechanisms. The first is that 7 mmol/L hypochlorite is capable of eliminating both Gram-negative and Gram-positive bacteria, and that the same concentration differentially degrades the function of IL-6 and IL-10. IL-6 was seen to be 3 times more susceptible to loss of function than IL-10 after only 5 minutes of exposure (P<0.005). The asymmetrical effect on these cytokines was observed between 7 and 1.75 mmol/L, and across the entire time scale examined. This hypochlorite formulation has the capability to sterilize a wound, and by altering the cytokine profile reduce inflammation and scarring, and improve wound healing.

Conclusions

These findings, taken with the burn survival study, suggest that the increased survival rate after application of hypochlorite could be due to the combined effect of elimination of infective pathogens, and the differential degradation of interleukin-6 and interleukin-10 at the site of injury
Periprocedural Use of Hypochlorous Acid Mist for Improving Healing and Cosmesis of the Face After Laser 2026-11-07 00:00

Marianna Blyumin-Karasik¹ | Jessica Colon² | Sophie Gaer¹ | Isabella Vigil¹ | Sylvie Nguyen² | Jordan Rosen¹

¹Precision Skin & Body Institute, Davie, Florida, USA |²Nova Southeastern University Dr. Kiran C. Patel College of Osteopathic Medicine, Davie, Florida, USA

Correspondence: Marianna Blyumin-Karasik (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。)

Received: 12 May 2025 | Revised: 7 August 2025 | Accepted: 12 August 2025

Funding: The authors received no specific funding for this work.

Keywords: aesthetic | cosmeceuticals | hypochlorous acid | integrated skincare | laser treatment | photoaging | resurfacing laser

ABSTRACT

Background: Laser resurfacing treatments have revolutionized dermatological procedures by improving skin texture, tone, and quality. Effective periprocedural care remains essential to reduce side effects, support healing, and optimize aesthetic outcomes. Hypochlorous acid (HOCl), a naturally occurring oxidant with anti-inflammatory and antimicrobial properties, has demonstrated efficacy in promoting wound healing and minimizing scarring.

Aim: To evaluate the use of stabilized HOCl mist in the periprocedural care of patients undergoing laser resurfacing.

Methods: Ten patients underwent treatment with UltraClear, CO2RE, and/or GentleMax Pro laser devices. Stabilized HOCl mist was applied before and after the procedure, with continued application twice daily for 1week posttreatment. Follow-up assessments at 1–3weeks and 1–3months post-procedure included clinical photography, tolerability evaluation, and aesthetic outcome assessment. Outcomes measured included Clinician Erythema Assessment (CEA), 4-point Edema Scale, Investigator Global Assessment of Pigmentation Scale (IPA), and Global Aesthetic Improvement Scale (GAIS). Statistical analysis was performed using the Friedman and Wilcoxon signed-rank tests.

Results: HOCl mist was associated with accelerated recovery, including a noticeable reduction in erythema and pigmentation. Statistically significant improvements were observed in CEA (p=0.007) and IPA (p=0.012) scores, indicating reduced clinical severity and pigment alteration. Edema and GAIS scores showed no statistically significant change.

Conclusion: Stabilized HOCl mist is well-tolerated and may significantly aid post-procedural recovery by minimizing side effects and reducing discomfort. Results support the potential role of stabilized HOCl mist as a beneficial adjunct in post-laser skincare, contributing to faster healing, reduced inflammation, and enhanced cosmetic results.
Effect of Sodium Hypochlorite 0.05% on MMP-9 Extracellular Release in Chronic Wounds 2026-11-06 00:00

Rosanna Maniscalco ¹, Giorgina Mangano ², Alessandra Capezzone de Joannon ^3,^*, Matteo Vergassola ³, Sara Zucchi ³, Ferdinando Mannello ¹ and Lorella Ragni ³

¹Unit of Clinical Biochemistry, Section of Biochemistry and Biotechnology, Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy

²Scientific Consultant, ToxHub S.r.l. Albano Laziale, 00073 Rome, Italy

³Global R&D Angelini Pharma S.p.A, Via Vecchia del Pinocchio, 22, 60131 Ancona, Italy

^*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Abstract: Background: In chronic wounds, high concentrations of matrix metalloproteinases (MMPs) can cause excessive proteolysis and slow wound healing. Consequently, restoring a proper MMP balance can help reduce the risk of a chronic wound. An antiseptic solution containing 0.05% sodium hypochlorite (Amukine Med 0.05%, Angelini S.p.A.; hereafter termed NaClO solution) is available on the market. The NaClO solution was proven effective and safe in managing infected skin wounds. To further characterize its activity, this study evaluated the in vitro activity of the NaClO solution on the monocyte release of MMPs. Methods: Human monocytic THP-1 (ATCC® TIB-202™) cell lines were differentiated into macrophages and treated with different concentrations of NaClO (from 0.05% to 5 × 10−7%). In addition, the THP-1 cell line was stimulated with wound fluid (WF) from patients with active venous leg ulcers in the inflammatory phase. The effect of NaClO (0.025–0.0062%) was also evaluated on healthy human peripheral blood serum samples. The effects of treatments on the gelatinolytic activity of MMP-9 were evaluated by gelatin zymography. The effects on MMPs release were evaluated through the Pro™ Human MMP 9-plex Assay. An exploratory scratch wound healing assay was also performed. Results: The NaClO solution reduced the gelatinolytic activity of MMP-9 and its activated form. The downregulation of MMP-9 gelatinolytic activity was also observed in peripheral blood serum. The MMPs profile showed a reduction in MMP-1 release (p < 0.05) and a slight reduction of the release of MMP-9 and MMP-12 after the treatment with LPS and the NaClO solution. A slight improvement in wound healing was observed after macrophage activation and treatment with the NaClO solution. Conclusions: The results obtained suggest a possible ability of the NaClO solution to modulate the proteolytic pathways in the wound microenvironment, further characterizing its activity and use in clinical practice during wound care.

Keywords: sodium hypochlorite; matrix metalloproteinases inhibition; gelatinolytic activity; chronic wound

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主任医师、教授、博导，南方医科大学第三附属医院（广东省骨科医院）院长

李孝建

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主任医师、教授、博导，南方医科大学第三附属医院（广东省骨科医院）院长

李孝建

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