Chun-Ju Chen 1 , Chun-Cheng Chen 2,3,* and Shinn-Jyh Ding 1,2,3,*
1Institute of Oral Science, Chung Shan Medical University, Taichung City 402, Taiwan; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
2School of Dentistry, Chung Shan Medical University, Taichung City 402, Taiwan
3Department of Dentistry, Chung Shan Medical University Hospital, Taichung City 402, Taiwan
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (C.-C.C.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (S.-J.D.);
Tel.: +886-6-2471-8668 (ext. 55255) (C.-C.C.); +886-6-2471-8668 (ext. 55529) (S.-J.D.)
Academic Editor: Ihtesham ur Rehman
Received: 13 April 2016; Accepted: 15 July 2016; Published: 19 July 2016
Abstract: Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability analysis was completed. Live/dead staining, morphology observation, alamarBlue assay, and lipopolysaccharide (LPS) detection were examined on grit-blasted and biofilm-contaminated titanium alloy discs after treatment with the three chemotherapeutic agents. The results indicated that HOCl exhibited better antibacterial efficacy with increasing irrigating volumes. HOCl achieved greater antibacterial efficacy as treatment time was increased. A decrease in antimicrobial effectiveness was observed when HOCl was unsealed and left in contact with the air. All the irrigants showed antibacterial activity and killed the majority of bacteria on the titanium alloy surfaces of biofilm-contaminated implants. Moreover, HOCl significantly lowered the LPS concentration of P. gingivalis when compared with NaOCl and CHX. Thus, a HOCl antiseptic may be effective for cleaning biofilm-contaminated implant surfaces.
Keywords: dental implant; hypochlorite acid; antiseptics; antimicrobial activity
Thomas H. Leung,1,2 Lillian F. Zhang,1 Jing Wang,1 Shoucheng Ning,3 Susan J. Knox,3 and Seung K. Kim1,4,5
1Department of Developmental Biology, 2Department of Dermatology, 3Department of Radiation Oncology, 4Howard Hughes Medical Institute, and 5Department of Medicine, Oncology Division, Stanford University School of Medicine, Stanford, California, USA.
Nuclear factor-κB (NF-κB) regulates cellular responses to inflammation and aging, and alterations in NF-κB signaling underlie the pathogenesis of multiple human diseases. Effective clinical therapeutics targeting this pathway remain unavailable. In primary human keratinocytes, we found that hypochlorite (HOCl) reversibly inhibited the expression of CCL2 and SOD2, two NF-κB–dependent genes. In cultured cells, HOCl inhibited the activity of inhibitor of NF-κB kinase (IKK), a key regulator of NF-κB activation, by oxidizing cysteine residues Cys114 and Cys115. In NF-κB reporter mice, topical HOCl reduced LPS-induced NF-κB signaling in skin. We further evaluated topical HOCl use in two mouse models of NF-κB–driven epidermal disease. For mice with acute radiation dermatitis, topical HOCl inhibited the expression of NF-κB–dependent genes, decreased disease severity, and prevented skin ulceration. In aged mice, topical HOCl attenuated age-dependent production of p16INK4a and expression of the DNA repair gene Rad50. Additionally, skin of aged HOCl-treated mice acquired enhanced epidermal thickness and proliferation, comparable to skin in juvenile animals. These data suggest that topical HOCl reduces NF-κB–mediated epidermal pathology in radiation dermatitis and skin aging through IKK modulation and motivate the exploration of HOCl use for clinical aims.
擅长:脊柱外科、脊柱微创技术、显微创伤外科、腰椎间盘突出症脊柱侧弯等。
熟练掌握了骨科常见病、多发病及疑难病症的诊治技术,成功抢救了各种多发伤、多发骨折等急诊、危重病人。能够熟练应用各种新型内固定材料手术治疗四肢及关节复杂骨折、复杂骨盆及髋臼骨折、骨不连等,手外伤的急诊手术、关节置换及翻修术、关节镜下手术,以及骨肿瘤、脊柱手术等各类骨科手术、手术治疗效果良好。能够熟练应用DHS、DCS、PFNA、髋动力锁定钢板、各种交锁髓内钉、解剖钢板及Liss钢板治疗四肢及关节复杂骨折、复杂骨盆及髋臼骨折手术、骨不连手术及手外伤的急诊手术、关节置换及翻修术、膝关节镜下半月板切除及交叉韧带重建术,以及骨肿瘤、脊柱手术等各类骨科手术、对创伤骨科微创治疗具有丰富的临床经验。
临床工作经验丰富,具有极为熟练的外科技能,在骨科各领域有较深的造诣。尤其在关节外科方面,已开展全身六大关节的关节镜检查和镜下手术,总体水平在广东领先,率先在广东开展关节镜下膝关节前、后交叉韧带重建术、膝关节半月板修补术、关节内骨折关节镜监视下复位固定术等高难度手术。并已开展四肢大关节的人工关节置换术,被广东省定为人工关节置换术指导专家。在复杂关节畸形的人工关节置换方面颇有研究。
擅长:危重烧伤救治/重度吸入性损伤的救治/小儿重度烧伤救治/严重皮肤撕脱伤及复合伤救治/烧伤瘢痕整复及功能重建/糖尿病足保肢治疗。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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